The elucidation of the role of human umbilical cord blood cells derived-exosomes in therapy for cerebral palsy
| Project/Area Number |
15K09716
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Kochi University |
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Principal Investigator |
Tsuda Masayuki 高知大学, 教育研究部医療学系基礎医学部門, 准教授 (90406182)
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| Co-Investigator(Kenkyū-buntansha) |
都留 英美 高知大学, 教育研究部医療学系基礎医学部門, 助教 (70380318)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 臍帯血 / エクソソーム / 脳性麻痺 |
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| Outline of Final Research Achievements |
Human umbilical cord blood (HUCB) cells are currently being used for autologous transplantation in the treatment of patients with cerebral palsy. However, it is unknown how HUCB cells, as a model of cell-based therapy, interact with brain parenchymal cells, and promote functional recovery. We hypothesized that HUBC cells communicate with parenchymal cells via exosomes. To investigate to the neuroprotective effects of HUCB cells derived-exosomes, we used the in vitro oxygen-glucose deprivation (OGD) model in SH-SY5Y cells. HUCB cells were exposed to the cerebral tissue extracts from mice subjected to hypoxic-ischemic encephalopathy (HIE). After 48hr culture, the exosome fractions were isolated and added to OGD SH-SY5Y cells culture. We found that HUCB cells derived-exosomes exposed HIE brain extracts significantly increased the viability of and attenuated the apoptosis of SH-SY5Y cells.
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Report
(4 results)
Research Products
(13 results)
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[Presentation] 新生仔脳虚血再灌流障害モデルマウスにおける組織障害とヒト臍帯血細胞移植によるサイトカイン・ケモカインの発現変化2016
Author(s)
馬場伸育, 王飛霏, 高石公子, 沈淵, 津田雅之, 山下竜幸, 都留英美, 飯塚美知郎, 柴垣里加子, 宮村充彦, 藤枝幹也, 前田長正, 相良祐輔
Organizer
第4回臍帯血による再生医療研究会学術集会
Place of Presentation
大阪国際会議場（大阪府大阪市）
Year and Date
2016-07-24
Related Report
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[Presentation] 新生仔脳虚血再灌流障害モデルマウスにおける内在性神経幹細胞の遊走評価2016
Author(s)
王飛霏, 沈淵, 山下竜幸, 馬場伸育, 都留英美, 高石公子, 飯塚美知郎, 柴垣里加子, 津田雅之, 宮村充彦, 藤枝幹也, 前田長正, 相良祐輔
Organizer
第4回臍帯血による再生医療研究会学術集会
Place of Presentation
大阪国際会議場（大阪府大阪市）
Year and Date
2016-07-24
Related Report
Invited
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[Presentation] 臍帯血幹細胞の未分化性維持培養の検討2016
Author(s)
都留英美, 津田雅之, 馬場伸育, 王飛霏, 山下竜幸, 沈淵, 相良祐輔, 前田長正
Organizer
第15回日本再生医療学会総会
Place of Presentation
大阪国際会議場（大阪市）
Year and Date
2016-03-17
Related Report
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