Hypothermia attenuate brain damage by coordinating the function of glia

• Project/Area Number: 15K09718
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Aichi Medical University
• Principal Investigator: Kakita Hiroki 愛知医科大学, 医学部, 講師 (40528949)
• Co-Investigator(Kenkyū-buntansha): 浅井 清文 名古屋市立大学, 大学院医学研究科, 教授 (70212462) ; 青山 峰芳 名古屋市立大学, 大学院薬学研究科, 教授 (70363918)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 新生児低酸素性虚血性脳症 / グリア / 低体温療法 / 一酸化窒素 / エリスロポエチン / サイトカイン / 低酸素性虚血性脳症 / 脳低温療法 / アストロサイト / ミクログリア

Outline of Final Research Achievements

Hypothermia has been reported to be only effective therapy for neonatal hypoxic ischemic encephalopathy (HIE) . We performed the examination of microenviroment improvement of glial function control with hypothermia. We found hypothermia (32-34℃) decreased the expression of iNOS and inflammatory cytokine in cultured glia under hypoxic condition. Furthermore, erythropoietin having neuroprotective effect made clear that overexpression lasted by a low temperature state. We found that these protein expression adjustment by hypothermia decreased brain damage. This study might contribute to the development of an etiology of HIE which is still a poor prognosis and the cure.

Research Products

• [Journal Article] Case report of Pierre Robin sequence with severe upper airway obstruction who was rescued by fiberoptic nasotracheal intubation. (2017)
  • Author(s): Satoru Takeshita, Hiroko Ueda, Tatenobu Goto, Hiroki Kakita, Kazuo Oshima, Takahisa Tainaka, Takayuki Ono, Yoshiaki Kazaoka, Yasumasa Yamada
  • Journal Title: BMC anesthesiology Volume: 17 Issue: 1 Pages: 43-43
  • DOI: 10.1186/s12871-017-0336-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Neuroprotective erythropoietin attenuates microglial activation, including morphological changes, phagocytosis, and cytokine production. (2017)
  • Author(s): Tamura T, Aoyama M, Ukai S, Kakita H, Sobue K, Asai K
  • Journal Title: Brain Res Volume: 1662 Pages: 65-74
  • DOI: 10.1016/j.brainres.2017.02.023
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CXCR4+CD45- cells are niche forming for osteoclastogenesis via the SDF-1, CXCL7, and CX3CL1 signaling pathways in bone marrow. (2016)
  • Author(s): Goto Y, Aoyama M, Sekiya T, Waguri-Nagaya Y, Miyazawa K, Asai K, Goto S.
  • Journal Title: Stem Cells Volume: 34 Issue: 11 Pages: 2733-2743
  • DOI: 10.1002/stem.2440
  • Peer Reviewed / Open Access
• [Journal Article] Fatal Tension Pneumopericardium in an Extremely Low Birth Weight Infant to Identify the Perforation Site of the Pericardium (2016)
  • Author(s): Daisuke Muto, Hiroki Kakita, Satoru Takeshita, Hiroko Ueda, Tatenobu Goto and Yasumasa Yamada
  • Journal Title: Pediatrics & Therapeutics Volume: 6 Issue: 01 Pages: 277-277
  • DOI: 10.4172/2161-0665.1000277
  • Peer Reviewed / Open Access
• [Journal Article] Neonatal Central Diabetes Insipidus Caused by Severe Perinatal Asphyxia (2016)
  • Author(s): Hiroko Ueda, Shingo Numoto, Hiroki Kakita, Satoru Takeshita, Daisuke Muto, Tatenobu Goto, Haruo Mizuno, Akihisa Okumura and Yasumasa Yamada
  • Journal Title: Pediatrics & Therapeutics Volume: 6 Issue: 01 Pages: 278-278
  • DOI: 10.4172/2161-0665.1000278
  • Peer Reviewed / Open Access
• [Journal Article] 高インスリン血性低血糖を合併した13トリソミーの症例 (2016)
  • Author(s): 佐藤 新紀子, 垣田 博樹, 今峰 浩貴
  • Journal Title: 日本新生児成育医学会雑誌 Volume: 28 Pages: 65-69
  • NAID: 40021166206
  • Peer Reviewed / Open Access
• [Presentation] エリスロポエチンのミクログリア活性抑制による神経保護作用 (2017)
  • Author(s): 垣田博樹、竹下覚、上田博子、近藤知子、山田恭聖
  • Organizer: 第５３回 日本周産期新生児学会
• [Presentation] 炎症性サイトカインTNFαによるアストロサイトから分泌される神経保護因 子エリスロポエチンの産生抑制メカニズムの解明 (2016)
  • Author(s): 垣田博樹、長屋嘉顕、竹下覚、上田博子、後藤盾信、 武藤大輔、山田恭聖
  • Organizer: 第６１回日本新生児成育医学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2016-12-01