| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We induced differentiation of iPS cells derived from healthy individuals into neuroectodermal cells. We then cultured these iPS cells in suspension cultures to obtain neuronal aggregates. Based on the shift of neuronal cell markers, we confirmed that these neuronal aggregates were emulating the cerebral cortex. When we exposed this fetal brain model to interleukin-6 (IL-6) for 24 hours, we observed increased levels of phosphorylated signal transducer and activator of transcription 3 (STAT3). Ten days later, we found increased levels of astrocytes and a reduction in neuronal cells. Simultaneous administration of luteolin downregulated the phosphorylation of STAT3 and inhibited the attenuation in neuronal differentiation induced by IL-6. We believe the neuronal system model used in this study can greatly contribute to the pathophysiological analysis and treatment discovery in fetal encephalopathy caused by the fetal environment.
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