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COL7A1 expression in the peripheral blood mononuclear cells

Research Project

Project/Area Number 15K09739
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionHirosaki University

Principal Investigator

NAKANO Hajime  弘前大学, 医学研究科, 准教授 (90281922)

Co-Investigator(Kenkyū-buntansha) 赤坂 英二郎  弘前大学, 医学部附属病院, 助教 (30436034)
六戸 大樹  弘前大学, 医学研究科, 助教 (50436036)
澤村 大輔  弘前大学, 医学研究科, 教授 (60196334)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords末梢血単核細胞 / VII型コラーゲン / 間葉系幹細胞 / Ⅶ型コラーゲン / 末梢血白血球
Outline of Final Research Achievements

We performed the isolation of COL7A1-expressing cells in the peripheral blood mononuclear cells. Culture of the peripheral blood mononuclear cells revealed that proliferation of the fusiform cells attached to the culture dish was observed. RT-PCR using total RNA extracted from the fusiform cells demonstrated that these cells expressed not only moderate COL7A1 expression but also weak CD45 and strong CD105 expressions. Immunofluorescence examinations showed that the fusiform cells were COL7A1+, CD29+, CD45-, CD34-, the same expression pattern as the mesenchymal stem cells. These findings strongly suggest that the mesenchymal stem cells circulate in the peripheral blood, which can be cultured as fusiform cells expressing COL7A1.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (27 results)

All 2017 2016 2015

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] Multiple milia as an isolated skin manifestation of dominant dystrophic epidermolysis bullosa: Evidence of phenotypic variability.2017

    • Author(s)
      Akasaka E, Nakano H, Takagi Y, Toyomaki Y, Sawamura D
    • Journal Title

      Pediatr Dermatol

      Volume: 34 Issue: 2

    • DOI

      10.1111/pde.13047

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An <i>ATP2A2</i> Missense Mutation in a Japanese Family with Darier Disease: A Case Report and Review of the Japanese Darier Disease Patients with <i>ATP2A2</i> Mutations2017

    • Author(s)
      Li M, Higashi N, Nakano H, Saeki H.
    • Journal Title

      Journal of Nippon Medical School

      Volume: 84 Issue: 5 Pages: 246-250

    • DOI

      10.1272/jnms.84.246

    • NAID

      130006206426

    • ISSN
      1345-4676, 1347-3409
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The first case of multiple pilomatricomas caused by somatic mutations of CTNNB1 without any associated disorder.2017

    • Author(s)
      Akasaka E, Nakano H, Fukui T, Korekawa A, Aizu T, Sawamura D
    • Journal Title

      J Dermatol Sci

      Volume: 88 Issue: 2 Pages: 259-260

    • DOI

      10.1016/j.jdermsci.2017.06.011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ER stress in the pathogenesis of pretibial dystrophic epidermolysis bullosa.2017

    • Author(s)
      Hattori M, Shimuzu A, Oikawa D, Kamei K, Kaira K, Ishida-Yamamoto A, Nakano H, Sawamura D, Tokunaga F, and Ishikawa O.
    • Journal Title

      Br. J. Dermatol.

      Volume: 印刷中 Issue: 4 Pages: e92-e93

    • DOI

      10.1111/bjd.15342

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] X-linked dominant protoporphyria: The first reported Japanese case.2016

    • Author(s)
      Ninomiya Y, Kokunai Y, Tanizaki H, Akasaka E, Nakano H, Moriwaki S.
    • Journal Title

      Journal of Dermatology

      Volume: 43 Issue: 4 Pages: 414-8

    • DOI

      10.1111/1346-8138.13101

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 優性栄養障害型表皮水疱症(痒疹型)VS結節性痒疹.2016

    • Author(s)
      赤坂英二郎、滝吉典子、中野 創、澤村大輔
    • Journal Title

      Visual Dermatol

      Volume: 15 Pages: 715-717

    • Related Report
      2016 Research-status Report
  • [Journal Article] Dystrophic epidermolysis bullosa pruriginosa successfully treated with immunosuppressants.2016

    • Author(s)
      Takahashi T, Mizutani Y, Ito M, Nakano H, Sawamura D, Seishima M
    • Journal Title

      J Dermatol

      Volume: 43 Issue: 11 Pages: 1391-1392

    • DOI

      10.1111/1346-8138.13406

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 足趾の爪病変で診断し、足底板使用にて管理良好な栄養障害型表皮水疱症2016

    • Author(s)
      新井 円, 高山 かおる, 中野 創, 横関 博雄
    • Journal Title

      皮膚科の臨床

      Volume: 58 Pages: 36-37

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Superficial epidermolytic ichthyosis caused by a novel KRT2 mutation.2015

    • Author(s)
      Akasaka E, Minakawa S, Rokunohe D, Toyomaki Y, Matsuzaki Y, Sawamura D, Nakano H
    • Journal Title

      J Dermatol Sci.

      Volume: 79 Issue: 1 Pages: 182-184

    • DOI

      10.1016/j.jdermsci.2015.04.006

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 末梢血間葉系幹細胞におけるⅦ型コラーゲン発現について2017

    • Author(s)
      赤坂英二郎、中野 創、神田由紀、鷹木由里子、豊巻由香、澤村大輔
    • Organizer
      第24回分子皮膚科学フォーラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Elevated serum IgE level, but not TARC and Eosinophil counts, may reflect the impairment of stratum corneum barrier function in healthy indivisuals; Results of cross-sectional study of 1137 Japanese healthy individuals.2017

    • Author(s)
      Akasaka E, Hara K, Takahashi M, Fukui T, Korekawa A, Nakano H, Takahashi I, Nakaji S, Sawamura D
    • Organizer
      76th Annual SID Meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 弘前大学皮膚科におけるファブリー病遺伝子診断の現状.2017

    • Author(s)
      中野 創
    • Organizer
      ファブリー病premiumシンポジウム in 東北
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] X連鎖性魚鱗癬の遺伝子診断について2017

    • Author(s)
      赤坂英二郎、中野 創、河野通浩、秋山真志、澤村大輔
    • Organizer
      第32回角化症研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ステロイドパルス両方に抵抗性を示し柴苓湯が奏功した後天性特発性全身性無汗症.2017

    • Author(s)
      赤坂英二郎、原 憲司、神 可代、中島康爾、会津隆幸、中野 創、澤村大輔
    • Organizer
      第4回汗と皮膚疾患の研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 帯状疱疹の診断と治療・分子標的薬による皮膚障害の対策2017

    • Author(s)
      中野 創
    • Organizer
      第245回学術生涯教育セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 遺伝性皮膚疾患の遺伝子診断2017

    • Author(s)
      中野 創
    • Organizer
      函館皮膚科医会学術講演
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 末梢血単核球由来mRNAを用いたCOL7A1変異検索の有用性について2016

    • Author(s)
      赤坂英二郎、中野 創、神田由起、鷹木由里子、豊巻由香、澤村大
    • Organizer
      日本皮膚科学会青森地方会第376回例会
    • Place of Presentation
      ホテル青森(青森県・青森市)
    • Year and Date
      2016-12-04
    • Related Report
      2016 Research-status Report
  • [Presentation] 栄養障害型表皮水疱症の遺伝子診断における末梢血単核球のⅦ型コラーゲン発現についての検討2016

    • Author(s)
      赤坂英二郎、中野 創、神田由起、鷹木由里子、豊巻由香、澤村大輔
    • Organizer
      第38回水疱症研究会
    • Place of Presentation
      ラフレさいたま(埼玉県・さいたま市)
    • Year and Date
      2016-11-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 骨髄性プロトポルフィリン症の症状差2016

    • Author(s)
      中野 創
    • Organizer
      第80回日本皮膚科学会東部支部学術大会
    • Place of Presentation
      アクトシティ浜松(静岡県・浜松市)
    • Year and Date
      2016-10-29
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] UROD遺伝子変異が同定された晩発性皮膚ポリフィリン症2016

    • Author(s)
      松井彰伸、赤坂英二郎、鷹木由里子、豊巻由香、神田由起、今 敦、原田 研、澤村大輔、中野 創
    • Organizer
      第38回日本光医学・光生物学会
    • Place of Presentation
      京都リサーチパークサイエンスホール(京都府・京都市)
    • Year and Date
      2016-07-22
    • Related Report
      2016 Research-status Report
  • [Presentation] 遺伝性皮膚ポルフィリン症の新知見2016

    • Author(s)
      中野 創
    • Organizer
      第115回日本皮膚科学会総会
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2016-06-03
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 栄養障害型表皮水疱症における末梢血単核球から分離したmRNAを用いたCOL7A1変異検索.2016

    • Author(s)
      赤坂英二郎、中野 創、神田由紀、鷹木由里子、豊巻由香、澤村大輔
    • Organizer
      第23回分子皮膚科学フォーラム
    • Place of Presentation
      ホテルグランヴィア大阪(大阪府・大阪市)
    • Year and Date
      2016-04-15
    • Related Report
      2016 Research-status Report
  • [Presentation] Mutational analysis of dystrophic epidermolysis bullosa.2016

    • Author(s)
      Akasaka E, Nakano H, Sawamura D
    • Organizer
      The 13th International Congress of Human Genetics
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2016-04-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 優性栄養障害型表皮水疱症の遺伝カウンセリング G2251E変異について2015

    • Author(s)
      赤坂 英二郎, 鷹木 由里子, 豊巻 由香, 澤村 大輔, 中野 創
    • Organizer
      第39回日本遺伝カウンセリング学会学術集会
    • Place of Presentation
      三井ガーデンホテル千葉(千葉県千葉市)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Research-status Report
  • [Presentation] シクロスポリンが奏功した優性栄養障害型表皮水疱症痒疹型の2例2015

    • Author(s)
      伊藤 満,安達由裕子,水谷陽子,周 円,加納宏行,中野創,清野真理子
    • Organizer
      第114回日本皮膚科学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-05-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Ⅶ型コラーゲン沈着が見られた優性栄養型表皮水疱症(前脛骨型)の1例.2015

    • Author(s)
      服部麻衣,清水 晶,加藤円香,天野博雄,山本明美,中野 創,澤村大輔,亀井希代子,徳永文稔,石川 治
    • Organizer
      第22回分子皮膚科学フォーラム
    • Place of Presentation
      ホテル日航高知旭ロイヤル(高知県高知市)
    • Year and Date
      2015-04-17
    • Related Report
      2015 Research-status Report
  • [Book] 皮膚科研修ノート2016

    • Author(s)
      中野 創、澤村大輔、宮地良樹、戸倉新樹、山本俊幸、山崎研志、佐山浩二、池田政身、多田譲治、盛山吉弘、伊藤周作、井上雄二、木花いずみ、江川清文、浅田秀夫、日野治子、渡辺大輔、清水 晶、川口博史、望月 隆
    • Total Pages
      712
    • Publisher
      診断と治療社
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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