Analysis of repair function of radiation-induced DNA damage on cultured human fibroblasts derived from patients with xeroderma pigmentosum
| Project/Area Number |
15K09751
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Osaka Medical College |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 色素性乾皮症 / 放射線 / DNA二本鎖切断 / γH2AH / 紫外線 / DNA損傷 / H2AX |
|---|
| Outline of Final Research Achievements |
Although radiation has been used to examine and sometimes treat patients with xeroderma pigmentosum(XP) and Cockayne syndrome (CS), radiation-induced DNA damage repair function in these patients has not been analyzed in detail. We investigated post-irradiation focus formation using IF staining with gannma-H2AX antibody, a highly sensitive marker of radiation-induced DNA damage (double-strand breaks;DSB) in skin fibroblasts from XP and CS patients. In CSB and XPV (not XPA and XPD/CS) cells, a decrease in focus formation was seen as compared with normal cells.These results suggest that repair of DSB in CS and XPV may be impaired. However, so fat the relation between decrease of DSB repair and XPV and CSB patients' phenotype /genotype was not obvious.
|
Report
(4 results)
Research Products
(17 results)
-
-
-
-
-
-
[Journal Article] Elevated urinary levels of 8-hydroxy-2’-deoxyguanosine in a Japanese child of xeroderma pigmentosum/Cockayne syndrome complex with Infantile onset of nephrotic syndrome2016
Author(s)
Kondo D, Noguchi A, Tamura H, Tsuchida S, Takahashi I, Kubota H, Yano T, Oyama C, Sawaishi Y, Moriwaki S, Takahashi T
-
Journal Title
Tohoki J Exp Med
Volume: 239
Issue: 3
Pages: 231-235
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] High prevalence of vitamin D deficiency in patients with xeroderma pigmetosum (XP)- A under strict sun-protection2015
Author(s)
Kuwabara A, Tsugawa N, Tanaka K, Uejima Y, Ogawa J, Otao N, Yamada N, Masaki T, Nishigori C, Moriwaki S, Okano T.
-
Journal Title
European Journal of Clinical Nutrition
Volume: Epub ahead of print
Issue: 6
Pages: 693-9
DOI
Related Report
Peer Reviewed
-
-
-
-
[Presentation] 色素性乾皮症BRUSHU2015
Author(s)
森脇真一
Organizer
第66回日本皮膚科学会中部支部学術大会
Place of Presentation
神戸国際会議場（兵庫県神戸市）
Year and Date
2015-11-01
Related Report
Invited
-
-
-
-
-
