| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Although radiation has been used to examine and sometimes treat patients with xeroderma pigmentosum(XP) and Cockayne syndrome (CS), radiation-induced DNA damage repair function in these patients has not been analyzed in detail. We investigated post-irradiation focus formation using IF staining with gannma-H2AX antibody, a highly sensitive marker of radiation-induced DNA damage (double-strand breaks;DSB) in skin fibroblasts from XP and CS patients. In CSB and XPV (not XPA and XPD/CS) cells, a decrease in focus formation was seen as compared with normal cells.These results suggest that repair of DSB in CS and XPV may be impaired. However, so fat the relation between decrease of DSB repair and XPV and CSB patients' phenotype /genotype was not obvious.
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