| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Outline of Final Research Achievements |
In floating culture of mouse ES cells, addition of bone morphogenetic protein 4 (BMP4) strongly suppress neural differentiation, while epidermal differentiation is not induced. In this study, we found that adherent culture of mouse ES cell aggregates on fibronectin led to epidermal differentiation. Comprehensive analysis by a DNA micro array revealed that the expression of ADAMTS-2 (Procollagen I N-protease) is significantly upregulated in the cells cultured on fibronectin with BMP4. The onset of ADAMTS-2 expression preceded that of epidermal stem cell genes such as CK14 and CK15, suggesting ADAMTS-2 plays a novel role in the determination of epidermal cell fate. We further performed epidermal differentiation of human ES cells, however, the methods developed in mouse ES cells was inapplicable to the human case. Thus, there may be different molecular mechanisms between mouse and human epidermal differentiation.
|
