| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We have been studying SCC on the basis of differentially expressed gene list generated by the combination of LCM and cDNA microarray technology. One transcription factor that was significantly upregulated in actinic keratosis and SCC compared to normal epidermis was E2F4. E2F4 is a member of the E2F family of transcription factors that have a critical role in the control of cellular proliferation and apoptosis. In this study, we aimed to elucidate the functions of E2F4 in development and progression of cutaneous SCC. We first evaluated the expression of E2F4 in various skin conditions by immunohistochemistry. The specific expression of E2F4 in the nucleus of SCC tissues but not in other skin conditions, such as basal cell carcinoma, seborrheic keratosis, and psoriasis was confirmed. E2F4 was detected in SCC cell lines (A431 and HSC-5) at mRNA and protein levels. Our results suggest that E2F4 may have important roles in development and progression of cutaneous SCC.
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