| Project/Area Number |
15K09788
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
TSUBOI RYOJI 東京医科大学, 医学部, 主任教授 (70221421)
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| Co-Investigator(Kenkyū-buntansha) |
原田 和俊 東京医科大学, 医学部, 准教授 (20324197)
山本 真実 (田中 真実 / 山本真実) 東京医科大学, 医学部, 助教 (60421062)
前 賢一郎 東京医科大学, 医学部, 助教 (60532257)
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAGUCHI MASAKIYO 岡山大学, 大学院医薬学総合研究科 細胞生物学, 准教授 (70379840)
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| Research Collaborator |
MAEDA TETSUO 東京医科大学, 医学部, 技術員
EGUSA CHIZU 東京医科大学, 医学部, 技術員
HIBINO TOSHIHIKO 資生堂, リサーチセンター
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | メラノーマ / 転移 / S100A8/9 / S100A8/A9 / 転移モデル / S100A9 / Emmprin / 転移抑制物質 |
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| Outline of Final Research Achievements |
The S100 protein family, especially S100A8 and S100A9, mediates the inflammatory reaction and is involved in the proliferation and metastasis of cancer cells. Emmprin (extracellular matrix metalloproteinase inducer) and neuroplastin b are S100A8/A9 receptors on the surface of keratinocytes. Immunohistological analysis of melanomas revealed that Emmprin was expressed at both the invasive edge of lesions and the adjacent skin. Tail vein-injected, highly Emmprin-expressing melanoma cells（SK-MEL2）metastasized to the skin of epidermis-specific S100A9 transgenic mice. In our recent study, a C57/BL6 mouse model of pulmonary metastasis from primary cutaneous melanoma (B16-F10 cells) was established to assess metastasis quantitatively by MRI. A model using S100A9 transgenic mice will be deployed to analyze metastasis of SK-MEL2 melanoma cells. ELISA screening failed to detect a molecule inhibiting the binding of S100A9 with recombinant Emmprin fixed on a plate.
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