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Role of Id protein in radioresponse of solid tumor

Research Project

Project/Area Number 15K09983
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Radiation science
Research InstitutionHokkaido University

Principal Investigator

Yasui Hironobu  北海道大学, アイソトープ総合センター, 准教授 (10570228)

Co-Investigator(Kenkyū-buntansha) 山盛 徹  北海道大学, 獣医学研究院, 准教授 (00512675)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords固形腫瘍 / 放射線 / Idタンパク質 / 細胞老化 / p53 / Inhibitor of DNA binding / 腫瘍 / 転写調節因子 / p21
Outline of Final Research Achievements

Recently, inhibitor of differentiation/DNA binding 1 (Id1) has been reported to play an important role in cellular processes such as proliferation and metastasis in cancer cells. However, the role of Id1 in the radioresistance of cancer cells remains unclear. In this study, knockdown of Id1 by its specific siRNA enhanced radiosensitivity of A549 cells expressing wild-type p53, but had little effect on HeLa or HT29 cells with aberrant p53 status. The population of senescent cells remarkably increased after X-irradiation in A549 cells, and this increase was enhanced by knockdown of Id1. X-ray-induced expression of p21, a key regulator for cellular senescence, was significantly higher in Id1-knockdowned cells compared with control cells. These results indicate that Id1 contributes to the radioresistance of cancer cells via downregulation of radiation-induced senescence, suggesting that Id1 is a potent target to increase the effectiveness of cancer radiotherapy in some types of cancer.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016 2015 Other

All Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 12 results,  Open Access: 8 results,  Acknowledgement Compliant: 4 results) Presentation (3 results) Remarks (4 results)

  • [Journal Article] Preclinical study on hypoxic radiosensitizing effects of glycididazole in comparison with those of doranidazole in vitro and in vivo.2018

    • Author(s)
      Yasui H, Kubota N, Nishizumi J, Sakai Y, Yamamori T, Inanami O.
    • Journal Title

      ONCOLOGY LETTERS

      Volume: 15 Pages: 1993-1998

    • DOI

      10.3892/ol.2017.7481

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ataxia-Telangiectasia Mutated (ATM) Kinase Regulates eNOS Expression and Modulates Radiosensitivity in Endothelial Cells Exposed to Ionizing Radiation2018

    • Author(s)
      Nagane Masaki、Kuppusamy M. Lakshmi、An Jennifer、Mast Jesse M.、Gogna Rajan、Yasui Hironobu、Yamamori Tohru、Inanami Osamu、Kuppusamy Periannan
    • Journal Title

      Radiation Research

      Volume: 189 Issue: 5 Pages: 519-528

    • DOI

      10.1667/rr14781.1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Metabolic analysis of radioresistant medulloblastoma stem-like clones and potential therapeutic targets2017

    • Author(s)
      Sun Lue、Moritake Takashi、Ito Kazuya、Matsumoto Yoshitaka、Yasui Hironobu、Nakagawa Hidehiko、Hirayama Aki、Inanami Osamu、Tsuboi Koji
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 4 Pages: 1-24

    • DOI

      10.1371/journal.pone.0176162

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantitative imaging of pO2 in orthotopic murine gliomas: hypoxia correlates with resistance to radiation2017

    • Author(s)
      Yasui Hironobu、Kawai Tatsuya、Matsumoto Shingo、Saito Keita、Devasahayam Nallathamby、Mitchell James B.、Camphausen Kevin、Inanami Osamu、Krishna Murali C.
    • Journal Title

      Free Radical Research

      Volume: 51 Issue: 9-10 Pages: 861-871

    • DOI

      10.1080/10715762.2017.1388506

    • NAID

      120006533133

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lipophilic tetraphenylphosphonium derivatives enhance radiation-induced cell killing via inhibition of mitochondrial energy metabolism in tumor cells2017

    • Author(s)
      Yasui H, Yamamoto K, Suzuki M, Sakai Y, Bo T, Nagane M, Nishimura E, Yamamori T, Yamasaki T, Yamada K, Inanami O
    • Journal Title

      Cancer Letters

      Volume: 390 Pages: 160-167

    • DOI

      10.1016/j.canlet.2017.01.006

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of the mechanism of radiation-induced upregulation of mitochondrial abundance in mouse fibroblasts.2016

    • Author(s)
      Yamamori T, Sasagawa T, Ichii O, Hiyoshi M, Bo T, Yasui H, Kon Y, Inanami O.
    • Journal Title

      Journal of Radiation Research

      Volume: - Issue: 3 Pages: 1-10

    • DOI

      10.1093/jrr/rrw113

    • NAID

      120006331861

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of the relative biological effectiveness of spot-scanning proton irradiation in vitro.2016

    • Author(s)
      Maeda K, Yasui H, Matsuura T, Suzuki M, Nam JM, Yamamori T, Inanami O, Shirato H.
    • Journal Title

      Journal of Radiation Research

      Volume: - Issue: 3 Pages: 307-311

    • DOI

      10.1093/jrr/rrv101

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of MPS1 inhibition on genotoxic stress responses in murine tumour cells.2016

    • Author(s)
      Suzuki M., Yamamori T., Yasui H., Inanami O.
    • Journal Title

      Anticancer Res.

      Volume: 36 Pages: 2783-2792

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A nucleoside anticancer drug, 1-(3-C-ethynyl-beta-D-ribo-pentofuranosyl)cytosine, induces depth-dependent enhancement of tumor cell death in spread-out bragg peak (SOBP) of proton beam.2016

    • Author(s)
      Maeda K, Yasui H, Yamamori T, Matsuura T, Takao S, Suzuki M, Matsuda A, Inanami O, Shirato H.
    • Journal Title

      PLoS One

      Volume: 11(11) Issue: 11 Pages: e0166848-e0166848

    • DOI

      10.1371/journal.pone.0166848

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inhibition of the mitochondrial fission protein dynamin-related protein 1 (Drp1) impairs mitochondrial fission and mitotic catastrophe after x-irradiation.2015

    • Author(s)
      Yamamori T., Ike S., Bo T., Sasagawa T., Sakai Y., Suzuki M., Yamamoto K., Nagane M., Yasui H., Inanami O.
    • Journal Title

      Mol. Biol. Cell.

      Volume: 26 Issue: 25 Pages: 4607-17

    • DOI

      10.1091/mbc.e15-03-0181

    • NAID

      120005677771

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Roles of ROS and PKC-betaII in ionizing radiation-induced eNOS activation in human vascular endothelial cells.2015

    • Author(s)
      Sakata K, Kondo T, Mizuno N, Shoji M, Yasui H, Yamamori T, Inanami O, Yokoo H, Yoshimura N, Hattori Y.
    • Journal Title

      Vascular Pharmacology

      Volume: - Pages: 55-65

    • DOI

      10.1016/j.vph.2015.03.016

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Downregulation of the DNA repair enzyme apurinic/apyrimidinic endonuclease 1 stimulates transforming growth factor-β1 production and promotes actin rearrangement.2015

    • Author(s)
      Sakai Y., Yamamori T., Yasui H., and Inanami O.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 印刷中 Issue: 1 Pages: 35-41

    • DOI

      10.1016/j.bbrc.2015.03.163

    • NAID

      120005757980

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 転写調節因子Id1はp53依存的に放射線誘発細胞老化を抑制する2017

    • Author(s)
      安井博宣,竹内麻依,山盛徹,松本英樹,高橋昭久,稲波修
    • Organizer
      第23回癌治療増感研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] がん細胞の放射線応答における転写調節因子Id1の役割2016

    • Author(s)
      安井博宣、竹内麻依、山盛徹、松本英樹、高橋昭久、稲波修
    • Organizer
      日本放射線影響学会第59回大会
    • Place of Presentation
      広島県広島市、JMSアステールプラザ
    • Related Report
      2016 Research-status Report
  • [Presentation] 転写調節因子Id1ノックダウンが放射線感受性に与える影響とそのメカニズムの解明2015

    • Author(s)
      竹内麻依、安井博宣、鈴木基史、酒井友里、山盛徹、稲波修
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      青森県十和田市、北里大学獣医学部
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Remarks] 北海道大学大学院獣医学研究院放射線学教室

    • URL

      https://www.vetmed.hokudai.ac.jp/organization/radbiol/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 北海道大学アイソトープ総合センター

    • URL

      https://www.hokudai.ac.jp/radiois/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 北海道大学大学院獣医学研究科放射線学教室

    • URL

      https://www.vetmed.hokudai.ac.jp/organization/radbiol/

    • Related Report
      2016 Research-status Report
  • [Remarks] 北海道大学アイソトープ総合センター

    • URL

      http://www.hokudai.ac.jp/radiois/kenkyusitu.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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