| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
In this study, the efficacy of some compounds was investigated to modify radiation or hyperthermia induced cell death. Lysolipin I or BU-4664L induced apoptosis in U937 and Molt-4 cells. In addition, Lisolipin I also enhanced radiation-induced apoptosis while, the low concentration BU-4664L have a protecting effect on radiation induced apoptosis. In this study, we determined that Tempo combine treatment with hyperthermia rapidly induced autophagic cell death. Tempo 5 mM -44℃/20 min combination induced apoptosis, while Tempo 5 mM -44℃/60 min combination induced autophagic cell death in HeLa cells. This co-treatment inhibited the processing of heat-activated procaspase-3 into active small subunits, leading to the inhibition of caspase-dependent apoptosis, and results in the induction of autophagic cell death. Furthermore, the gene chip analysis showed the up-regulation of TP53INP1 and cyclin-dependent kinase inhibitor (CDKI) genes in the combination treatment.
|
