| Project/Area Number |
15K10007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kitasato University |
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Principal Investigator |
Kagiya Go 北里大学, 医療衛生学部, 准教授 (30524243)
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| Co-Investigator(Kenkyū-buntansha) |
小川 良平 富山大学, 大学院医学薬学研究部(医学), 准教授 (60334736)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUMOTO HIDEKI 福井大学, 高エネルギー医学研究センター, 准教授 (40142377)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | アポトーシス / がん幹細胞 / 低酸素細胞 / イメージング / 可視化 / がんがん幹細胞 / プロテインスプライシング / FRET |
|---|
| Outline of Final Research Achievements |
Cancer stem cells (CSCs) residing in hypoxic regions as their niche are considered responsible for metastasis and recurrence after therapeutic treatment. Thus, a non-invasive and real-time imaging system to monitor apoptosis of cells in tumor hypoxic regions would be a useful tool to develop and evaluate a novel chemoradiation therapy targeting the CSCs. We have succeeded in improvement of an imaging system for hypoxic cell apoptosis, taking advantage of a cyclic enzyme of a shrimp-derived luciferase (Luc), detecting more than 350-times higher luminescence from cells with apoptosis induction under the hypoxic condition compared to our previous system with a cyclic firefly-derived Luc under the identical condition. This improvement should be a big progress to establish an imaging system to firmly detecting CSC apoptosis.
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