| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Outline of Final Research Achievements |
The heavy-ion irradiation is used as a radiation therapy, although DNA repair mechanism is not fully understood. In this research, the number of DNA double strand breaks or the foci of DNA repair proteins were analyzed after X-ray or heavy-ion irradiation with various linear energy transter in mammalian cells where specific DNA repair pathways were inhibited by the lack of DNA repair proteins genetically or the pretreatment of inhibitors against DNA repair proteins. The main repair pathway for DNA double strand breaks after both X-ray or heavy-ion irradiation was non-homologous end joining. In addition, homologous recombination could complement the defect of non-homologous end joining after X-ray irradaition, but not after high energy heavy-ion irradiation completely.
|
