| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The effect of focal mechanical stress on the localization of TRPV2 was investigated in HT1080 cells. When focal mechanical stress was applied, subplasma membrane Ca2+ concentration ([Ca2+]s) was increased beneath the pipette, which propagated throughout the cell. The increase in [Ca2+]s was blocked by knocking down TRPV2. The focal mechanical stress induced vinculin conformation change was blocked by knocking down TRPV2. Elevation of [Ca2+]s was not observed by the application of Gd3+ and mechanical stress induced accumulation of TRPV2 -GFP beneath the pipette was not observed.
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