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Development of the methods to fabricate a macro-scale liver tissue containinf the bile duct usgin 3D bioprinter

Research Project

Project/Area Number 15K10028
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionKyushu University (2015, 2017)
Fukuoka University (2016)

Principal Investigator

Yanagi Yusuke  九州大学, 大学病院, 助教 (30596664)

Co-Investigator(Kenkyū-buntansha) 田口 智章  九州大学, 医学研究院, 教授 (20197247)
中山 功一  佐賀大学, 医学部, 特任教授 (50420609)
林田 真  九州大学, 大学病院, その他 (70452761)
松浦 俊治  九州大学, 大学病院, 助教 (10532856)
孝橋 賢一  九州大学, 医学研究院, 講師 (10529879)
Co-Investigator(Renkei-kenkyūsha) YAMAZA Takayoshi  九州大学, 歯学(系)研究科(研究院), 准教授 (80304814)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords肝再生 / 肝組織工学 / 細胞凝集反応 / 再生医療 / Tissue engineering / 人工肝臓 / 肝組織構築
Outline of Final Research Achievements

We established the original culture protocol to reproducively create the hepatic spheroids which contained human mature hepatocytes, HUVEC, BM-MSC. These spheroids showed high function and could be manipulate stablely by the 3D bioprinter. We developed the large device to fix the spherooids and improved the cerculating culture system to construct effectively a larger hepatic tissue using the 3D bioprinter. Following these works, we established the methods to fabricate the hepatic tissue several times as large as previous one. We cultured the hepatic tissues which contained mature hepatocytes,HUVECs and BM-MSC for 1 month. As a result, the tissue showed vascular formation and biliaty constructs by the histlogical examination.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] In vivo and ex vivo methods of growing a liver bud through tissue connection2017

    • Author(s)
      Yanagi Yusuke、Nakayama Koichi、Taguchi Tomoaki、Enosawa Shin、Tamura Tadashi、Yoshimaru Koichiro、Matsuura Toshiharu、Hayashida Makoto、Kohashi Kenichi、Oda Yoshinao、Yamaza Takayoshi、Kobayashi Eiji
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 14085-14085

    • DOI

      10.1038/s41598-017-14542-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] バイオ3Dプリンターを用いたscaffold-free細胞構造体による小児外科領域に再生医療研究2017

    • Author(s)
      栁 佑典
    • Organizer
      第16回 日本再生医療学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] バイオ3Dプリンターを用いたscaffold-free細胞構造体による小児外科領域に再生医療研究2017

    • Author(s)
      栁 佑典
    • Organizer
      第16回 日本再生医療学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Biofabrication of a transplantable macroscale liver tissue using a 3D bio-printer2017

    • Author(s)
      Yusuk Yanagi
    • Organizer
      International Pediatric Transplantation Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 乳歯幹細胞を用いた肝再生研究の現状と展望2016

    • Author(s)
      栁 佑典
    • Organizer
      第52回日本周産期・新生児医学会学術集会
    • Place of Presentation
      富山
    • Year and Date
      2016-07-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 肝オルガノイド移植2016

    • Author(s)
      栁 佑典
    • Organizer
      第34回日本肝移植研究会
    • Place of Presentation
      旭川
    • Year and Date
      2016-07-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 乳歯肝細胞を用いた肝再生医療研究2016

    • Author(s)
      栁 佑典
    • Organizer
      第53回日本小児外科学会
    • Place of Presentation
      福岡
    • Year and Date
      2016-05-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 肝組織移植を目指した肝組織構築と移植法の検討2016

    • Author(s)
      柳 佑典
    • Organizer
      第15回日本再生医療学会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report
  • [Presentation] A new strategy for liver tissue fabrication and transplantation.2016

    • Author(s)
      Yusuke Yanagi
    • Organizer
      The 25th conference of APASL (Asian Pacific Association for the Study of the Liver)
    • Place of Presentation
      Tokyo
    • Year and Date
      2016-02-20
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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