New strategies for treatment of the gastrointestinal stromal tumor by the high sensitivity DNA mutation diagnostic assay
| Project/Area Number |
15K10038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤田 逸郎 日本医科大学, 医学部, 講師 (10287740)
松谷 毅 日本医科大学, 医学部, 准教授 (50366712)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 消化管間質腫瘍 |
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| Outline of Final Research Achievements |
The gastrointestinal stromal tumor (GIST) arising under the mucosa has difficulty in clinical diagnosis unlike the diagnosis of the cancer. Aim of the research is to develope the new assay that can identify the specific DNA mutant of GIST in the mixed sample of tumor and normal cells.
After various condition tests by basic experiment, we developed high sensitivity assay that became able to identify specific mutation.
The assay was finally applied to to a very small amount of samples obtained from the clinical specimen, and the specific DNA mutation in the gene of GIST was successfully detected by the assay.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で微量な試料から抽出したDNAを用いて消化管間質腫瘍の特定の遺伝子変異をとらえることが可能となる高感度な変異遺伝子同定法を開発した。臨床診断困難な消化管間質腫瘍を旧来から行われていた顕微鏡検査による病理診断ではなく、DNA変異をとらえることで遺伝子による診断が可能となると考える。
本手法の臨床応用により消化管間質腫瘍の診断能向上が期待され、新たな消化管間質腫瘍の治療戦略に結びつけることが可能になると考える。
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Report
(5 results)
Research Products
(5 results)
