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One-Step Nucleic Acid Amplification Assay for Detection of Axillary Lymph Node Metastases in Breast Cancer Patients Treated with Neoadjuvant Chemotherapy

Research Project

Project/Area Number 15K10051
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionOsaka University

Principal Investigator

Shimazu Kenzo  大阪大学, 医学系研究科, 講師 (30448039)

Research Collaborator TAKAMOTO Kaori  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords乳癌 / センチネルリンパ節生検 / 術前化学療法 / OSNA / センチネルリンパ節 / 術中迅速診断法 / サイトケラチン19
Outline of Final Research Achievements

Sentinel lymph nodes (SLNs), and non-SLNs from 88 breast cancer patients treated with NAC were examined by means of histology and OSNA. The diagnostic accuracy, sensitivity, and specificity of OSNA for were 87.8, 75.0, and 91.2 % respectively for SLNs, and the corresponding values were and 95.1, 97.3, and 94.6 % for non-SLNs. The diagnostic accuracy of OSNA was significantly lower for SLNs than for non-SLNs (P = 0.021), which was attributable to the low sensitivity for detection of micrometastases (micromets) due to lower CK19 mRNA expression detected by in situ hybridization (ISH) in SLN micromets than in non-SLN micromets. For primary breast tumors, CK19 mRNA expression showed a significant reduction after NAC (P = 0.040). The findings obtained with CK19 mRNA ISH suggest that most SLN micromets cannot be detected by OSNA due to the reduced expression of CK19 mRNA induced by NAC.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] One-Step Nucleic Acid Amplification Assay for Detection of Axillary Lymph Node Metastases in Breast Cancer Patients Treated with Neoadjuvant Chemotherapy.2016

    • Author(s)
      Takamoto K, Shimazu K, Naoi Y, Shimomura A, Shimoda M, Kagara N, Kim SJ, Tamaki Y, Noguchi S.
    • Journal Title

      Annals of Surgical Oncology

      Volume: 23(1) Issue: 1 Pages: 78-86

    • DOI

      10.1245/s10434-015-4693-y

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Construction of a nomogram, based on one-step nucleic acid amplification, for prediction of non-sentinel node metastasis in breast cancer patients with sentinel node metastasis2017

    • Author(s)
      佐藤信昭
    • Organizer
      16th St. Gallen International Breast Cancer Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] OSNA法を用いたノモグラムによる乳癌ノンセンチネルリンパ 節転移術中診断2017

    • Author(s)
      荻谷 朗子
    • Organizer
      第25回日本乳癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 術前化学療法後のセンチネルリンパ節転移診断におけるOSNA法の有用性について2015

    • Author(s)
      高本香 島津研三
    • Organizer
      第23回日本乳癌学会学術総会
    • Place of Presentation
      東京国際フォーラム(東京都千代田区)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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