| Project/Area Number |
15K10062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Sakaguchi Koichi 京都府立医科大学, 医学(系)研究科(研究院), 講師 (00405284)
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| Co-Investigator(Kenkyū-buntansha) |
田口 哲也 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80243260)
原田 義規 京都府立医科大学, 医学(系)研究科(研究院), 講師 (10381956)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 早期乳癌 / センチネルリンパ節 / 5-ALA / PpIX / 乳癌 / 5アミノレブリン酸 |
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| Outline of Final Research Achievements |
A total of 10 surgery patients with early breast cancer were enrolled in this study. After 5-ALA was applied orally 3 h prior to surgery, 25 SLNs were subjected to spectral fluorescence imaging, intraoperative frozen section diagnosis, histopathological diagnosis, and statistical analysis was performed. No apparent side effect associated with 5-ALA administration was observed. The spectral fluorescence intensity of PpIX in metastatic SLNs was greater than that in nonmetastaic SLNs. This photodynamic method was able to obtain sensitivity of over 90% and a false negative rate of less than 5% accuracy comparison to both intraoperative frozen section diagnosis and histopathological diagnosis. Our results show the potential of 5-ALA-induced PpIX fluorescence processed by photodynamic technique for detecting metastases in SLNs from patients with breast cancer, suggesting that this rapid and feasible method can be applicable to diagnosis of resected SLN samples in pathology laboratories.
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