• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Chemosensitivity and microRNA signature in esophageal cancer

Research Project

Project/Area Number 15K10084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionTohoku University

Principal Investigator

Saiki Yuriko  東北大学, 医学系研究科, 助教 (80311223)

Co-Investigator(Kenkyū-buntansha) 小川 武則  東北大学, 大学病院, 講師 (50375060)
Co-Investigator(Renkei-kenkyūsha) Takeuchi Hiroya  慶應義塾大学, 医学部, 准教授 (20265838)
Research Collaborator Sunamura Makoto  東北大学, 医学系研究科, 講師 (10201584)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsesophageal cancer / microRNA / chemosensitivity / ABCB1 / esophagel cancer / head and neck cancer / タキサン耐性 / エピジェネティクス / マイクロRNA
Outline of Final Research Achievements

We established taxane resistant esophageal cancer cell lines and explored possible mechanisms for the acquisition of chemoresistance. We found that the ABCB1 gene encoding a member of the ATP binding cassette (ABC) transporter proteins was significantly upregulated in taxane resistant esophageal cancer cell lines. Moreover, ABCB1 amplification was found in taxane resistant cells. In addition, after treatment with 5-Aza and/or TSA in parental TE1 cell line, ABCB1 was upregulated; epigenetic mechanism may be involved. Analyses of CpG islands within the downstream promoter demonstrated that taxane resistant cells showed unmethylated CGI whereas parental cells were dominantly methylated. In conclusion, ABCB1 gene amplification alongside with alteration in epigenetic mechanism could be responsible for acquisition of taxane resistance in esophageal cancer cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Surgical technique for allogeneic uterus transplantation in macaques2016

    • Author(s)
      Obara H, Kisu I, Kato Y, Yamada Y, Matsubara K, Emoto K, Adachi M, Matoba Y, Umene K, Nogami Y, Banno K, Tsuchiya H, Itagaki I, Kawamoto I, Nakagawa T, Ishigak Hi, Itoh Y, Ogasawara K, Saiki Y, Sato S-i, Nakagawa N, Shiina T, Aoki D, Kitagawa Y
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 35989-35989

    • DOI

      10.1038/srep35989

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ABCB1 in side populations is a key ABC transporter essential for acquired taxane-chemoresistance in esophageal cancer cells2015

    • Author(s)
      齋木由利子
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際展示場(名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi