Clinical application of candidate stem cell markers for detection of circulating tumor cells in esophageal cancer.
| Project/Area Number |
15K10088
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
嶋田 裕 京都大学, 医学(系)研究科(研究院), 講師 (30216072)
塚田 一博 富山大学, 大学院医学薬学研究部(医学), 教授 (90171967)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | cancer stem cells / esophageal cancer / circulating tumor cells / 末梢血液循環癌細胞 / 食道癌 / 胃癌 / 幹細胞 / 癌幹細胞マーカー |
|---|
| Outline of Final Research Achievements |
We isolated and characterized quiescent cancer stem cells (CSCs) population in esophageal squamous cell carcinoma (ESCC). We isolated p75NTR-positive cells in the G0-G1 phase but not in the S-G2-M phase showed strong expression of stem cell-related genes; high colony formation ability; high tumorigenicity in a mouse xenograft model; and strong chemoresistance, suggesting that p75NTR-positive/G0-1 cells represent quiescent CSCs in ESCC. For diagnostic application , our two-color flow cytometric investigation using peripheral blood samples of ESCC patients revealed that p75NTR-positive, but not p75NTR-negative circulating tumor stem cell counts, correlated with clinically diagnosed distant metastasis and pathological venous invasion in ESCC tumors. Further investigation targetting CSCs which are specifically characterized by multiple markers may provide us with novel therapeutic and diagnostic strategies for patients with ESCC.
|
Report
(4 results)
Research Products
(15 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Detection of circulating tumor cells by the expression of a cancer stem cell marker CD271 (p75NTR) in patients with esophageal cancer2015
Author(s)
Tomoyuki Okumura, Tetsuji Yamaguch, Katsuhisa Hirano, Toru Watanabe, Hirohumi Kojima, Isaya Hashimoto, Kazuto Shibuya, Shozo Hojo, Koshi Matsui, Isaku Yoshioka, Takuya NagataYutaka Shimada, Kazuhiro Tsukada,
Organizer
The 74thAnnual Meeting of the Japanese Cancer Association
Place of Presentation
Nagoya, Japan
Year and Date
2015-10-08
Related Report
-
[Presentation] Current status and challenges in surgical treatment of esophageal cancer2015
Author(s)
Okumura T, Kawai S, Miwa T, Yamaguch T, Hirano K, Watanabe T, Kojima H, Moriyama M, Sekine S, Hashimoto I, Shibuya K, Hojo S, Matsui K, Yoshioka I, Nagata T, Tsukada K
Organizer
The International Forum on MicroManufacturing & Biofabrication 2015
Place of Presentation
Toyama, Japan
Year and Date
2015-05-18
Related Report
Int'l Joint Research / Invited
-
