Establishment of epigenome panel for identification of lymph node metastasis in gastric cancer

• Project/Area Number: 15K10094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Mie University
• Principal Investigator: Yasuda Hiromi 三重大学, 医学部附属病院, 助教 (60586767)
• Co-Investigator(Kenkyū-buntansha): 問山 裕二 三重大学, 医学部附属病院, 講師 (00422824) ; 毛利 靖彦 三重大学, 医学系研究科, 客員准教授 (70345974) ; 楠 正人 三重大学, 医学系研究科, 教授 (50192026)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 胃癌 / リンパ節転移 / 遠隔転移 / microRNA / DNAメチル化 / メチル化

Outline of Final Research Achievements

The aim of this study was to identify clinically relevant epigenetic markers for presence of lymph node metastasis in gastric cancer. Array-based comprehensive analysis demosntrated that miR-4286 expression was significantly increased with disease development and lymph node metastasis in gastric cancer. Although we could not identify approproate candidates of methylated DNA, gene expression analysis demonstrated that increased ANGPTL2 expression was depply involved in lymph node metastasis and poor prognosis in gastric cancer patients.

Research Products

• [Journal Article] Clinical impact of preoperative albumin to globulin ratio in gastric cancer patients with curative intent. (2017)
  • Author(s): Toiyama Y, Yasuda H, Ohi M, Yoshiyama S, Araki T, Tanaka K, Inoue Y, Mohri Y, Kusunoki M.
  • Journal Title: Am J Surg Volume: 213 Issue: 1 Pages: 120-126
  • DOI: 10.1016/j.amjsurg.2016.05.012
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Burden of C-Reactive Protein/Albumin Ratio Before Curative Surgery for Patients with Gastric Cancer. (2016)
  • Author(s): Toiyama Y, Shimura T, Yasuda H, Fujikawa H, Okita Y, Kobayashi M, Ohi M, Yoshiyama S, Hiro J, Araki T, Inoue Y, Mohri Y, Kusunoki M.
  • Journal Title: Anticancer Res. Volume: 36 Pages: 6491-6498
  • Peer Reviewed
• [Journal Article] Circulating microRNA-203 predicts metastases, early recurrence, and poor prognosis in human gastric cancer. (2015)
  • Author(s): Imaoka H, Toiyama Y, Okigami M, Yasuda H, Saigusa S, Ohi M, Tanaka K, Inoue Y, Mohri Y, Kusunoki M
  • Journal Title: Gastric cancer Volume: 2 Aug
  • NAID: 40020881612
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 胃癌におけるCSF-1/CSF-1R共発現の予後マーカーとしての有用性と癌進展における機序 (2017)
  • Author(s): 奥川 喜永
  • Organizer: 日本癌治療学会学術集会
• [Presentation] 消化器癌腹膜播種に対する新たなアプローチ 消化管悪性腫瘍におけるBDNF/TrkB axisの腹膜播種進展における機序解明と、治療標的としての可能性 (2017)
  • Author(s): 奥川 喜永
  • Organizer: 日本消化器病学会
• [Presentation] 胃癌におけるAZIN1 RNA修飾とADAR1/ADAR2発現の臨床的意義 (2017)
  • Author(s): 奥川 喜永
  • Organizer: 日本癌学会総会
• [Presentation] ステージ2、3胃癌における予後因子の検討 (2017)
  • Author(s): 安田 裕美
  • Organizer: 日本胃癌学会
• [Presentation] Circulating microRNA-203 Predicts Metastases, Early Recurrence, and Poor Prognosis in Human Gastric Cancer (2015)
  • Author(s): Yuji Toiyama, Hiroki Imaoka, Masato Okigami, Hiromi Yasuda, Susumu Saigusa, Masaki Ohi, Minako Kobayashi, Toshimitsu Araki, Koji Tanaka, Yasuhiro Inoue, Yasuhiko Mohri, Masato Kusunoki
  • Organizer: DDW
  • Place of Presentation: USA（ワシントンDC）
  • Year and Date: 2015-05-17
  • Int'l Joint Research