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Monitoring the HER2 copy number status in circulating tumor DNA by droplet digital PCR in patients with gastric cancer

Research Project

Project/Area Number 15K10110
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

Kazuma Okamoto  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (20285258)

Co-Investigator(Kenkyū-buntansha) 市川 大輔  山梨大学, 大学院総合研究部, 教授 (20347446)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsリキッドバイオプシー / 胃癌 / HER2 / バイオマーカー / 胃十二指腸外科学 / ddPCR / 遊離DNA
Outline of Final Research Achievements

Oncogenic activation (e.g. amplification) of uman epidermal growth factor receptor 2 (HER2) results in its protein overexpression and subsequent abnormal cell signalling, which contributes to cancer progression. Important issues for HER2-targeted therapy in GC are inaccurate assessments of HER2 status owing to intratumoral heterogeneity, which results in inappropriately choosing patients for HER2-targeted therapy. We investigated the potential clinical utility of the droplet digital PCR (ddPCR)-based HER2 copy number (CN) as a marker for the temporal and/or spatial heterogeneities of GC during treatment progress. The plasma HER2 amplification determined by ddPCR is a repeatable and noninvasive approach for real- time evaluations of the HER2 status to monitor the effects of treatments for patients with HER2-positive GC and enable treatment options for patients with HER2-negative GC but positive conversion of the HER2 status after recurrence.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] Monitoring the HER2 copy number status in circulating tumor DNA by droplet digital PCR in patients with gastric cancer.2017

    • Author(s)
      Shoda K, Ichikawa D, Fujita Y, Masuda K, Hiramoto H, Hamada J, Arita T, Konishi H, Komatsu S, Shiozaki A, Kakihara N, Okamoto K, Taniguchi H, Imoto I, Otsuji E.
    • Journal Title

      Gastric Cancer

      Volume: 1 Issue: 1 Pages: 126-135

    • DOI

      10.1007/s10120-016-0599-z

    • NAID

      40021062596

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Clinical utility of circulating cell-free Epstein-Barr virus DNA in patients with gastric cancer.2017

    • Author(s)
      Shoda K, Ichikawa D, Fujita Y, Masuda K, Hiramoto H, Hamada J, Arita T, Konishi H, Kosuga T, Komatsu S, Shiozaki A, Okamoto K, Imoto I, Otsuji E.
    • Journal Title

      Oncotarget

      Volume: 17 Issue: 17 Pages: 28796-28804

    • DOI

      10.18632/oncotarget.15675

    • NAID

      120006306639

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 胃癌ゲノム情報を考慮したtailor-made liquid biopsy2018

    • Author(s)
      Shoda K, Okamoto K, Ichikawa D, Fujita Y, Masuda K, Hiramoto H, Hamada J, Arita T, Konishi H, Komatsu S, Shiozaki A, Kakihara N, Taniguchi H, Imoto I, Otsuji E.
    • Organizer
      消化器外科学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Droplet digital PCRを用いた胃癌患者血漿遊離DNAにおけるHER2増幅モニタリング2016

    • Author(s)
      庄田勝俊 市川大輔 岡本和真
    • Organizer
      消化器癌発生学会
    • Place of Presentation
      城山観光ホテル
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 末梢循環遊離DNAを用いた血漿 HER2 増幅診断法の開発と胃癌治療における有用性の検討2016

    • Author(s)
      市川大輔 岡本和真
    • Organizer
      日本外科学会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2016 Research-status Report
  • [Presentation] 胃癌患者循環遊離DNAを用いた新たな血漿HER2増幅診断法の開発と有用性の検討2015

    • Author(s)
      市川大輔
    • Organizer
      第23回消化器関連学会週間
    • Place of Presentation
      グランドプリンスホテル新高輪(東京都港区)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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