Development of the new treatment strategy targeting microRNA which controls histone de-methylase

• Project/Area Number: 15K10142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kumamoto University
• Principal Investigator: SAKAMOTO Yasuo 熊本大学, 医学部附属病院, 非常勤診療医師 (00452897)
• Co-Investigator(Kenkyū-buntansha): 馬場 祥史 熊本大学, 医学部附属病院, 特任講師 (20599708) ; 宮本 裕士 熊本大学, 医学部附属病院, 講師 (80551259)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: JMJD3 / microRNA / EZH2 / 大腸癌 / mir186-5p

Outline of Final Research Achievements

We identified microRNA, miR186-5p which suppress the expression of histone de-methylase JMJD3 with enzyme activity opposite to EZH2. We confirmed that the suppression of JMJD3, CDH1, VASH1, and TFAP2E by the over-expression of miR186-5p in colon cancer cell line. We confirmed that cisplatin sensitivity was increased when I controlled expression of histone methylase EZH2 in colon cancer cell line.

Research Products

• [Journal Article] The Prognostic Significance of Histone Lysine Demethylase JMJD3/KDM6B in Colorectal Cancer. (2016)
  • Author(s): Tokunaga R, Sakamoto Y, Nakagawa S, Miyake K, Izumi D, Kosumi K, Taki K, Higashi T, Imamura Y, Ishimoto T, Iwatsuki M, Baba Y, Miyamoto Y, Yoshida N, Oki E, Watanabe M, Baba H.
  • Journal Title: Ann Surg Oncol Volume: 23(2) Issue: 2 Pages: 678-85
  • DOI: 10.1245/s10434-015-4879-3
  • Peer Reviewed
• [Journal Article] Retroileal colorectal anastomosis after extended left colectomy: application for laparoscopic surgery. (2016)
  • Author(s): Sakamoto Y, Tokunaga R, Miyamoto Y, Ohuchi M, Nakamura K, Kosumi K, Harada K, Shigaki H, Kurashige J, Iwatsuki M, Baba Y, Yoshida N, Baba H.
  • Journal Title: Surg Today. Volume: 46(12) Issue: 12 Pages: 1476-1478
  • DOI: 10.1007/s00595-016-1313-9
  • Peer Reviewed
• [Presentation] 腹腔鏡下前方切除術における縫合不全予防のための工夫 (2017)
  • Author(s): 坂本快郎、大内繭子、内原智幸、問端 輔、八木泰佑、中村健一、美馬浩介、澤山 浩、木下浩一、岩槻政晃、馬場祥史、吉田直矢、馬場秀夫
  • Organizer: 第86回大腸癌研究会
• [Presentation] 内臓肥満が大腸癌に与える影響: Leptinは女性の大腸癌予後を悪化させる (2017)
  • Author(s): 宮本裕士、大内繭子、坂本快郎、徳永竜馬、織田枝里、澤山 浩、岩槻政晃、馬場祥史、吉田直矢、馬場秀夫
  • Organizer: 第72回日本消化器外科学会総会
• [Presentation] 大腸癌切除症例において術後合併症が予後に与える影響 (2017)
  • Author(s): 宮本裕士、日吉幸晴、坂本快郎、澤山 浩、岩槻政晃、馬場祥史、吉田直矢、馬場秀夫
  • Organizer: JDDW 2017