Development of alleviation of rejection by autologous cardiogenic stem cell from explanted heart

• Project/Area Number: 15K10216
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Okayama University
• Principal Investigator: Arai Sadahiko 岡山大学, 医歯薬学総合研究科, 講師 (50345617)
• Co-Investigator(Renkei-kenkyūsha): OH Hidemasa 岡山大学病院, 新医療研究開発センター, 教授 (50372579)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医療 / 心臓移植 / 心臓血管外科

Outline of Final Research Achievements

We evaluated effect of autologous cardiogenic stem cell by heterotopic cardiac transplantation model in rat. We observed tendency of prolongation of graft survival and improved cardiac function in data. Further evaluation and statistic analysis are on-going. We analyzed origin of cardiac cell in myocardium. We found mixed existence of myocardial cell and cardiogenic stem cell. At this moment we cannot conclude whether the effect of cardiogenic stem cell is based on regeneration and proliferation of myocardium from stem cell or paracrine function from stem cell by endocrine hormone and chemical mediators and so on. Thus we continue further morphological evaluation of myocardial tissue of transplanted graft, we also considering further evaluations based on hormone/ chmical mediator analysis.

Research Products

• [Journal Article] Intracoronary cardiac progenitor cells in single ventricle phsiology The PERSEUS (Cardiac progenitor cell infusion to treat univentricular heart disease) randomized phase 2 trial (2017)
  • Author(s): S Ishigami, S Ohtsuk, T Eitoku, D Ousaka, M Kondo, Y Kurita, K Hirai, Y Fukushima, K Baba, T Goto, N Horio, J Kobayashi, Y Kuroko, Y Kotani, S Arai, T Iwasaki, S Sato, S Kasahara, S Sano, H Oh
  • Journal Title: Circulation research Volume: 120 Issue: 7 Pages: 1162-1173
  • DOI: 10.1161/circresaha.116.310253
  • Peer Reviewed / Open Access
• [Journal Article] Surgical outcome of hypoplastic left heart syndrome with intact atrial septum (2015)
  • Author(s): Arai S, Fujii Y, Kotani Y, Kuroko Y, Kasahara S, Sano S
  • Journal Title: Asian Cardiovasc Thoac Ann. Volume: 23 Pages: 1034-38
  • Peer Reviewed / Open Access
• [Journal Article] Intracoronary autologous cardiac progeitor cell transfer in patient with hypoplastic left heart syndrome: ths TICAP prospective phase 1 controlled trial (2015)
  • Author(s): Ishigami S, Ohtsuki S, Tarui S, Ousaka D, Kondo M, Okuyama M, Kobayashi J, Baba K, Arai S, Kawabata T, Yosihizumi K, Tateishi A, Kuroko Y, Iwasaki T, Sato S, Kasahara S, Sano S, Oh H
  • Journal Title: Circulation Research Volume: 116 Pages: 653-64
• [Presentation] 単心室症における自己心臓内幹細胞治療の臨床と先天性心疾患におけるiPS細胞の研究の現況と展望 (2017)
  • Author(s): 新井禎彦、石神修大、小林純子、後藤拓弥、佐野俊和、大月審一、王 英正、小谷恭弘、黒子洋介、笠原真悟、佐野俊二
  • Organizer: 第53回日本小児循環器学会
  • Invited