| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Our goal is to create a whole beating heart fabricated on an organ scaffold for human heart transplantation. We successfully performed the first heterotopic transplantation of a decellularized whole porcine heart with mesenchymal stem cells. The porcine heart was harvested following cardiac arrest induced by a high-potassium solution and stored at -80℃ for 24 hours. The porcine heart was completely decellularized and sterilized with gamma irradiation. Cultured mesenchymal stem cells were collected and either infused into the ascending aorta or injected directly into the left ventricular wall. Finally, recellularized whole-heart scaffolds were transplanted into pigs under systemic anti-coagulation treatment with heparin. Coronary artery angiography of the transplanted heart graft was performed. The scaffolds endured surgical procedures, and we detected short-term coronary artery perfusion in the transplanted scaffolds by angiography.
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