Donor-derived cell-free DNA is associated with acute rejection and decreased oxygenation in primary graft dysfunction after living donor-lobar lung transplantation
| Project/Area Number |
15K10256
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory surgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OTO Takahiro 岡山大学, 大学病院, 教授 (40452578)
TOYOOKA Shinichi 岡山大学, 大学院医歯薬学総合研究科, 教授 (30397880)
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| Research Collaborator |
TANAKA Shin
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 肺移植 / 生体肺移植 / 一塩基多型 / 血中遊離DNA / 原発性移植片不全 / 急性拒絶反応 / 診断 / デジタルPCR / DNA |
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| Outline of Final Research Achievements |
Donor-derived cell-free DNA (dd-cf-DNA) has been shown to be an informative biomarker of rejection after lung transplantation (LT) from deceased donors. However, in living-donor lobar LT (LDLLT), because small grafts are implanted with short ischemic time from blood-relatives, the detection of dd-cf-DNA might be challenging. Our study was aimed to examine the role of dd-cf-DNA in the early phase after LDLLT. Immediately after LDLLT, the dd-cf-DNA levels were highly elevated, subsequently reaching the plateau, with the resolution of primary graft dysfunction (PGD). Increased levels of dd-cf-DNA significantly correlated with decreased oxygenation immediately and 72 hours after LDLLT. The dd-cf-DNA levels significantly increased in the patients with acute rejection than in those with infection or stable condition. The measurement of dd-cf-DNA might be useful to monitor the severity of PGD, and dd-cf-DNA could be a potential biomarker for the diagnosis of acute rejection after LDLLT.
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Report
(4 results)
Research Products
(12 results)
