Research and development of a new therapy for lung carcinoma focused on miR-143.

• Project/Area Number: 15K10271
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Tokyo Medical University
• Principal Investigator: Matsubayashi Jun 東京医科大学, 医学部, 准教授 (00338790)
• Co-Investigator(Kenkyū-buntansha): 大平 達夫 東京医科大学, 医学部, 教授 (40317847) ; 池田 徳彦 東京医科大学, 医学部, 主任教授 (70246205) ; 高梨 正勝 東京医科大学, 医学部, 講師 (80312007) ; 岩館 寛大 山陽小野田市立山口東京理科大学, 工学部, 准教授 (70279107)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 肺腺癌 / miRNA / KRAS / ヒト肺腺癌 / miR-143 / 3’-UTR / 粘液産生性腺癌 / MAPK

Outline of Final Research Achievements

In our study, we focused on lung adenocarcinoma with the KRAS mutation to identify a new therapy for KRAS-mutated lung adenocarcinoma using miRNA to target KRAS. We examined the miRNAs correlated to the 3’-untranslated region of KRAS from the miRNA database. Among these miRNAs, we focused on four in particular, including miR-143. We analyzed the expressions of KRAS and these miRNAs in a human adenocarcinoma cell line by transfection of each of the four miRNAs mimics. Although the expression of KRAS in the lung adenocarcinoma cell line was not knocked down by transfection of any of these mimics, the expression was knocked down by transfection of a mixture of the four mimics.

Research Products

• [Journal Article] Prognostic factors for surgically resected non-small cell lung cancer with cavity formation. (2018)
  • Author(s): Shigefuku Shunsuke、Kudo Yujin、Yunaiyama Daisuke、Matsubayashi Jun、Park Jinho、Nagao Toshitaka、Shimada Yoshihisa、Saji Hisashi、Hagiwara Masaru、Okano Tetsuya、Kakihana Masatoshi、Kajiwara Naohiro、Ohira Tatsuo、Ikeda Norihiko
  • Journal Title: Journal of Thoracic Disease Volume: 10 Pages: 973-983
  • DOI: 10.21037/jtd.2018.01.61
  • Peer Reviewed
• [Journal Article] Tumor volume determines the feasibility of cell-free DNA sequencing for mutation detection in non-small cell lung cancer. (2016)
  • Author(s): Ohira T, Sakai K, Matsubayashi J, Kajiwara N, Kakihana M, Hagiwara M, Hibi M, Yoshida K, Maeda J, Ohtani K, Nagao T, Nishio K, Ikeda N.
  • Journal Title: Cancer Sci. Volume: 107 Pages: 1660-1666
  • Peer Reviewed
• [Journal Article] The Frequency and Prognostic Impact of Pathological Microscopic Vascular Invasion According to Tumor Size in Non-Small Cell Lung Cancer. (2016)
  • Author(s): Shimada Y, Saji H, Kato Y, Kudo Y, Maeda J, Yoshida K, Hagiwara M, Matsubayashi J, Kakihana M, Kajiwara N, Ohira T, Ikeda N.
  • Journal Title: Chest. Volume: 149 Pages: 775-785
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Association between high-resolution computed tomography findings and the IASLC/ATS/ERS classification of small lung adenocarcinomas in Japanese patients. (2015)
  • Author(s): Kudo Y, Matsubayashi J, Saji H, Akata S, Shimada Y, Kato Y, Kakihana M, Kajiwara N, Ohira T, Nagao T, Ikeda N.
  • Journal Title: Lung Cancer. Volume: 90 Pages: 47-54
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Correlation between whole tumor size and solid component size on high-resolution computed tomography in the prediction of the degree of pathologic malignancy and the prognostic outcome in primary lung adenocarcinoma. (2015)
  • Author(s): Saji H, Matsubayashi J, Akata S, Shimada Y, Kato Y, Kudo Y, Nagao T, Park J, Kakihana M, Kajiwara N, Ohira T, Ikeda N.
  • Journal Title: Acta Radiol. Volume: 56 Pages: 1187-1195
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prognostic Factors for Survival After Recurrence in Patients With Completely Resected Lung Adenocarcinoma: Important Roles of Epidermal Growth Factor Receptor Mutation Status and the Current Staging System. (2015)
  • Author(s): Kudo Y, Shimada Y, Saji H, Kato Y, Yoshida K, Matsubayashi J, Nagase S, Kakihana M, Kajiwara N, Ohira T, Nagao T, Ikeda N.
  • Journal Title: Clin Lung Cancer. Volume: 16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Factors Influencing the Diagnostic Accuracy of Identifying the Histologic Type of Non-Small-Lung Cancer With Small Samples. (2015)
  • Author(s): Oikawa T, Ohira T, Matsubayashi J, Konaka C, Ikeda N.
  • Journal Title: Clin Lung Cancer. Volume: 16 Pages: 374-378
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 肺がんの新分類と予後 肺がん新分類と予後 臨床データの比較と応用 (2017)
  • Author(s): 前田純一、濱中和嘉子、吉田浩一、垣花昌俊、梶原直央、大平達夫、三宅真司、松林純、長尾俊孝、池田徳彦
  • Organizer: 日本臨床細胞学会
• [Presentation] 比較的早期の肺癌患者由来cell free DNAの体細胞遺伝子変異検出率の臨床病理学的規定因子の検討 (2017)
  • Author(s): 坂井和子、西尾和人、大平達夫、松林純、梶原直央、垣花昌俊、萩原優、吉田浩一、前田純一、大谷圭志、池田徳彦、長尾俊孝
  • Organizer: 日本肺癌学会
• [Presentation] 肺多形癌のCT形態の検討 (2016)
  • Author(s): 眞田 知英, 朴 辰浩, 齋藤 和博, 徳植 公一, 池田 徳彦, 松林 純, 長尾 俊孝
  • Organizer: 日本肺癌学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-12-19
• [Presentation] Liquid Biopsy 効率的な遺伝子診断を目指して 肺癌手術患者に対するリキッドバイオプシーの有用性と限界 (2016)
  • Author(s): 大平 達夫, 坂井 和子, 前原 幸夫, 前田 純一, 吉田 浩一, 萩原 優, 垣花 昌俊, 岡野 哲也, 松林 純, 梶原 直央, 長尾 俊孝, 西尾 和人, 池田 徳彦
  • Organizer: 日本肺癌学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-12-19
• [Presentation] 肺原発リンパ上皮腫様癌の1例 (2016)
  • Author(s): 礒本 愛子, 松林 純, 山科 光正, 三宅 真司, 山口 真由実, 前田 純一, 垣花 昌俊, 大平 達夫, 池田 徳彦, 長尾 俊孝
  • Organizer: 日本臨床細胞学会春期大会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-05-27
• [Presentation] 浸潤性粘液産生性腺癌における術後再発の検討 (2016)
  • Author(s): 江里口 大介, 大谷 圭志, 工藤 勇人, 松林 純, 萩原 優, 岡野 哲也, 垣花 昌俊, 梶原 直央, 大平 達夫, 池田 徳彦
  • Organizer: 日本呼吸器外科学会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-05-12
• [Presentation] すりガラス成分を有する肺腺癌における3D-CT画像と組織学的脈管侵襲の関係 (2016)
  • Author(s): 田中 健彦, 吉田 浩一, 工藤 勇人, 朴 辰浩, 松林 純, 牧野 洋二郎, 荒牧 直, 萩原 優, 岡野 哲也, 垣花 昌俊, 梶原 直央, 大平 達夫, 池田 徳彦
  • Organizer: 日本呼吸器外科学会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-05-12
• [Presentation] 間質性肺炎合併肺癌における画像所見及び臨床像と病理所見の整合性に関する検討. (2015)
  • Author(s): 荒牧 直，嶋田 善久，松林 純，川本 雅司，朴 辰浩，吉村 明修，赤田 壮一，長尾 俊孝，徳植 公一，池田 徳彦.
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2015-11-28
• [Presentation] 浸潤性粘液産生性腺癌切除例のCT所見とその特徴 (2015)
  • Author(s): 江里口大介，工藤 勇人，嶋田 善久，松林 純，山田 隆文，朴 辰浩，大澤潤一郎，加藤 靖文，垣花 昌俊，前田 純一，吉田 浩一，萩原 優，梶原 直央，大平 達夫，池田 徳彦.
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2015-11-27
• [Presentation] 小型肺腺癌におけるHRCT所見とIASLC/ATS/ERS分類を用いた臨床病理学的因子の関係 (2015)
  • Author(s): 工藤 勇人，松林 純，赤田 壮一，嶋田 善久，雨宮 亮介，加藤 靖文，垣花 昌俊，梶原 直央，大平 達夫，池田 徳彦.
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2015-11-26
• [Presentation] 開胸時胸腔細胞診陽性症例の臨床病理学的検討 (2015)
  • Author(s): 垣花 昌俊，名和 公敏，前田 純一，吉田 浩一，萩原 優，長瀬 清亮，加藤 靖文，松林 純，梶原 直央，大平 達夫，長尾 俊孝，池田 徳彦.
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2015-11-26
• [Presentation] 肺腺癌におけるWHO新分類に基づいた細胞像 (2015)
  • Author(s): 三宅 真司，松林 純，前田 純一，垣花 昌俊，大平 達夫，池田 徳彦，長尾 俊孝.
  • Organizer: 第54回日本臨床細胞学会秋期大会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-11-22
• [Presentation] Impact of lymph node involvement on survival in patients with completely resected pulmonary squamous cell carcinoma. (2015)
  • Author(s): Kudo Y, Shimada Y, Amemiya R, Maeda J, Yoshida K, Kato Y, Hagiwara M, Matsubayashi J, Kakihana M, Kajiwara N, Ohira T, Ikeda N.
  • Organizer: 16th World Conference on Lung Cancer.
  • Place of Presentation: Denver, USA
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] Prognostic factors for survival after recurrence in patients with completely resected lung adnocarcinoma. (2015)
  • Author(s): Kudo Y, Shimada Y, Kato Y, Yoshida K, Matsubayashi J, Nagase S, Kakihana M, Kajiwara N, Ohira T, Nagao T, Ikeda N.
  • Organizer: The 61st Annual Congress of International College of Surgeons Japan Section
  • Place of Presentation: Tokyo
  • Year and Date: 2015-06-20
  • Int'l Joint Research
• [Presentation] Correlation between high-resolution computed tomography. findings and IASLC/ATS/ERS classification of small lung adenocarcinomas in Japanese patients (2015)
  • Author(s): Kudo Y, Matsubayashi J, Shimada Y, Akata S, Kato Y, Kakihana M, Kajiwara N, Ohira T, Nagao T, Ikeda N.
  • Organizer: 2015 The American Society of Clinical Oncology (ASCO) Annual Meeting.
  • Place of Presentation: Chikago, USA
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Book] 細胞診ガイドライン 4 呼吸器・胸腺・体腔液・リンパ節 (2015)
  • Author(s): 松林純 他多数
  • Total Pages: 264
  • Publisher: 金原出版