Functional analysis of candidate genes for oncogenesis in thymic carcinoma and establishment of individualized therapy

• Project/Area Number: 15K10279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Nagoya City University
• Principal Investigator: Okuda Katsuhiro 名古屋市立大学, 大学院医学研究科, 准教授 (50529170)
• Co-Investigator(Kenkyū-buntansha): 矢野 智紀 名古屋市立大学, 大学院医学研究科, 研究員 (40315883) ; 森山 悟 名古屋市立大学, 大学院医学研究科, 研究員 (50551264)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ウイルスベクター / Gateway system / KIT / Tyrosine kinase domain / gene mutation / PD-L1 / ベクター作成

Outline of Final Research Achievements

We carried out functional analysis on 25 candidate genes of oncogenic driver mutation by using nest generation sequencer. Functional analysis was performed on KIT gene mutation, which is a potential candidate gene of oncogenic driver mutation, but it suggested that just only the KIT gene mutation may not have carcinogenicity. In addition, five tyrosine kinase domain mutations were analyzed for surgical cases of thymic carcinoma and lung cancer, but no mutation was observed.
We revealed that the positive cases of PD-L1 protein expression in thymic carcinoma is relatively high and immune checkpoint inhibitors can be an effective treatment for thymic carcinoma.

Research Products

• [Journal Article] A comparative study of PD-L1 immunohistochemical assays with four reliable antibodies in thymic carcinoma. (2018)
  • Author(s): Sakane T, Murase T, Okuda K, Takino H, Masaki A, Oda R, Watanabe T, Kawano O, Haneda H, Moriyama S, Saito Y, Yamada T, Nakanishi R, Inagaki H.
  • Journal Title: Oncotarget Volume: 9 Pages: 6993-7009
  • Peer Reviewed / Open Access
• [Journal Article] Expression of excision repair cross-complementation group 1 and class Ⅲ β-tubulin in thymic carcinoma. (2017)
  • Author(s): Okuda K, Suzuki A, Tatematsu T, Haneda H, Moriyama S, Yano M, Nakanishi R.
  • Journal Title: Oncol Lett. Volume: 13 Pages: 3144-3150
  • Peer Reviewed / Open Access
• [Journal Article] Expression of excision repair cross-complementation group 1 and class Ⅲ β-tubulin in thymic carcinoma (2017)
  • Author(s): Okuda K, Oda R, Suzuki A, Tatematsu T, Handeda H, Moriyama S, Yano M, Nakanishi R.
  • Journal Title: Oncology Letters Volume: 13 Pages: 3144-3150
  • Peer Reviewed / Open Access
• [Journal Article] A case of mediastinum undifferentiated high grade pleomorphic sarcoma (2015)
  • Author(s): Okuda K, Yano M, Moriyama S, Haneda H, Tatematsu T, Suzuki A, Oda R, Nakanishi R
  • Journal Title: International Journal of Clinical and Experimental Medicine Volume: 8 Pages: 19566-70
  • Peer Reviewed / Open Access
• [Presentation] 胸腺癌におけるPD-L1蛋白の発現 (2018)
  • Author(s): 坂根理司、奥田勝裕、渡邊拓弥、小田梨紗、横田圭右、羽田裕司、森山悟、中西良一
  • Organizer: 第118回日本外科学会定期学術集会
• [Presentation] Treatment Outcomes of Primary Malignant Germ Cell Tumors of the Mediastinum (2017)
  • Author(s): Sakane T, Moriyama S, Haneda H, Okuda K, Kawano O, Watanabe T, Oda R, Nakanishi R
  • Organizer: 18th World Conference on Lung Cancer
  • Int'l Joint Research
• [Presentation] 胸腺癌における癌精巣抗原の発現 (2017)
  • Author(s): 坂根理司、鈴木あゆみ、小田梨紗、川野理、奥田勝裕、羽田裕司、森山悟、中西良一
  • Organizer: 第34回日本呼吸器外科学会総会
• [Presentation] Expression of excision repair cross complementation group 1 and class Ⅲ β-tubulin in thymic carcinoma (2015)
  • Author(s): Okuda K, Yano M, Moriyama S, Haneda H, Tatematsu T, Suzuki A, Oda R, Nakanishi R
  • Organizer: 16thWorld Conference on Lung Cancer
  • Place of Presentation: Colorado Convention Center, Denver, U.S.A.
  • Year and Date: 2015-09-08
  • Int'l Joint Research