Combined therapy for brain infarction with slow releasing drug delivery system and immature cells.

• Project/Area Number: 15K10291
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: University of Tsukuba
• Principal Investigator: Tsurushima Hideo 筑波大学, 医学医療系, 准教授 (50315470)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 脳梗塞 / 幹細胞治療 / Drug delivery system / 脂肪細胞由来幹細胞 / 脳梗塞治療 / 再生医療 / 脂肪由来幹細胞 / DDS / 生体内細胞分化コントロール / 脱分化脂肪細胞 / 幹細胞

Outline of Final Research Achievements

1)Brain infarction rats were treated using adipose-cell derived stem cells (ACSC), which were injected intra-arterially. Results: The mortality of ACSC-treated rats was lower than that of control rats. In survived brain infarction rats, infarction area of ASCS-treated rats was significantly decreased than that of control rats. Neurological examination was also improved in ASCS-treated rats. Hitological examination showed the existence of ACSC in brain infarction area.
2)Drug delivery system slowly releasing cytokines to central nervous system was developed. Cytokines were loaded on burr hole button that was used in cranioplasty as bone filling biomaterials. In-vivo slow releasing of cytokines was recognized from this burr hole button.

Research Products

• [Journal Article] Neurovascular unit protection from cerebral ischemia-reperfusion injury by radical-containing nanoparticle in mice. (2017)
  • Author(s): Hosoo H, Marushima A, Nagasaki Y, Hirayama A, Ito H, Puentes S, Mujagic A, Tsurushima H, Tsuruta W, Suzuki K, Matsui H, Matsumaru Y, Yamamoto T, Matsumura A.
  • Journal Title: Stroke Volume: 48(8) Pages: 2238-2247
  • Peer Reviewed / Open Access
• [Journal Article] Calcium phosphate nanoparticles prepared from infusion fluids for stem cell tramsfection: process optimization and cytotoxicity analysis. (2017)
  • Author(s): Shubhra QTH, Oyane A, Araki H, Nakamura M, Tsurushima H.
  • Journal Title: Biomater Sci. Volume: 5(5) Pages: 972-981
  • Peer Reviewed / Open Access
• [Journal Article] Hybrid Assistive Limb enhances the gait fuctions in sub-acute stroke stage: A multi single-case study. (2016)
  • Author(s): Yoshikawa K, Mizukami M, Kawamoto H, Sano A, Koseki K, Hashizume Y, Asakawa Y, Iwamoto K, Kohno Y, Nagata H, Nakai K, Tsurushima H
  • Journal Title: Physiotherapy Practice Research Volume: 37 Pages: 91-100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gait training of subacute stroke patients using a hybrid assiative limb: a pilot study. (2016)
  • Author(s): Mizukami M, Yoshikawa K, Kawamoto H, Sano A, Koseki K, Asakwa Y, Iwamoto K, Nagata H, Tsurushima H, Nakai K, Marushima A, Sankai Y, Matsumura A
  • Journal Title: Disabil. Rehabil. Assist Technol. Volume: Mar. 26 Pages: 1-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Controlled superficial assembly of DNA-amorphous calcium phosphate nanocomposite spheres for surface-mediated gene delivery. (2016)
  • Author(s): Oyane A, Araki H, Nakamura M, Shimizu Y, Shubhra QT, Ito A, Tsurushima H
  • Journal Title: B Biointerfaces Volume: 1 Pages: 519-527
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Controlled superficial assembly of DNA-amorphous calcium phosphate nanocomposite spheres for surface-mediated gene delivery. (2016)
  • Author(s): Oyane A, Araki H, Nakamura M, Shimizu Y, Shubhra QT, Ito A, Tsurushima H,
  • Journal Title: Colloids Surf. B Biointerfaces. Volume: 1 Pages: 519-527
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Area-specific cell stimulateon via surface-mediated gene transfer using apatite-based composite layers. (2015)
  • Author(s): Yazaki Y, Oyane A, Sogo Y, Yamazaki A, Tsurushima H,
  • Journal Title: Int. J. Mol. Sci. Volume: 16 Pages: 8294-8309
  • Peer Reviewed / Int'l Joint Research
• [Patent(Industrial Property Rights)] 分散性リン酸カルシウムナノ粒子 (2017)
  • Inventor(s): 中村真紀、大矢根綾子、鶴嶋英夫、他
  • Industrial Property Rights Holder: 中村真紀、大矢根綾子、鶴嶋英夫、他
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017