The roles of miRNAs in ischemic preconditioning

• Project/Area Number: 15K10296
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: University of Yamanashi
• Principal Investigator: KINOUCHI Hiroyuki 山梨大学, 大学院総合研究部, 教授 (30241623)
• Co-Investigator(Kenkyū-buntansha): 吉岡 秀幸 山梨大学, 大学院総合研究部, 助教 (20402076) ; 若井 卓馬 山梨大学, 大学院総合研究部, 医学研究員 (30456446)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 虚血性神経細胞障害 / 虚血耐性 / microRNA / 脳梗塞 / 虚血性神経細胞傷害 / micro RNA

Outline of Final Research Achievements

microRNAs (miRNAs) are a class of small, non-coding RNAs which mediate post-transcriptional regulation of gene expression. On the other hand, ischemic preconditioning involves numerous gene expression changes; however, its mechanism is still obscure. In this study, we evaluated the roles of miRNAs in ischemic preconditioning using rats and PC12 cells. Our results show that the expressions of a part of miRNAs were modified in the preconditioned brains or cells although further studies are necessary to clarify the relationship between miRNAs and gene expression changes.