| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Brain generates a micromolar order of carbon monoxide (CO) via heme oxygenase (HO) reactions. In this study, we examined the hypothesis that the deletion of HO-2 exacerbates cerebral energy metabolism during acute focal brain ischemia. We conducted quantitative imaging mass spectrometry (Q-IMS) analysis for adenylates and lactate to decipher local cerebral responses of energy metabolism from wild-type (WT) and HO-2-null mice that underwent a 60-min occlusion of the left middle cerebral artery. Striking differences were found in the contralateral hemispheres, where the levels of ADP, AMP and lactate in HO-2-null mice were higher than those in WT mice. These results suggest that the HO-2/CO system plays roles in protecting against ischemia not only at the ischemic core where the severe reduction of blood flow occurs, but it also protects even more effectively in the trans-hemispheric regions where only a subtle reduction of blood flow takes place.
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