| Project/Area Number |
15K10369
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
西川 亮 埼玉医科大学, 医学部, 教授 (90237678)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | DNA / glioma / pediatric / CSF / circulating / genetic |
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| Outline of Final Research Achievements |
Using cerebrospinal fluid of glioma patients, tumor-derived circulating cell free (ccf) DNA was extracted. It was found that more than 50 ng of ccf DNA can be obtained from 1 ml of cerebrospinal fluid. Using this DNA as a template, it was possible to screen point mutations of IDH1, H3F3A, BRAF genes by real-time PCR / high resolution melting curve analysis method. Moreover, it was also found that quantitative analysis of MGMT gene promoter methylation is also possible.
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