| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In the rat with intervertebral disc degeneration model, M1 macrophages infiltrated from 1 to 2 weeks after injury, and M2 macrophages appeared 2 weeks after injury and gradually decreased. In the disc 8 weeks after injury, only M2 macrophages existed at the portion of outer layer of degenerated annuls fibrosus. The macrophages that appeared 1 week after injury including M2 macrophages at 8 weeks were demonstrated that were of marrow origin by the GFP positive chimeric rats.
In the degenerated human intervertebral discs that were harvested by anterior decompression surgery of cervical spine, the granulation tissue with rich newly developed blood vessels contained a lot of macrophages, and also included appearance of neurotrophic factors and nerve fibers with its receptors. On the other hand, the granulation tissue with a lot of M2 macrophages include less blood vessels and nerve fibers.
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