| Project/Area Number |
15K10396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
内山 茂晴 信州大学, 医学部, 特任教授 (10242679)
加藤 博之 信州大学, 学術研究院医学系, 教授 (40204490)
植村 一貴 信州大学, 医学部附属病院, 特任研究員 (50624706)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 腱細胞 / マイクロRNA / Myostatin / 筋芽細胞株 / 標的遺伝子 / GDF8 / miRNA / Smad7 / Tppp3 / ALK-smad2/3シグナル |
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| Outline of Final Research Achievements |
Recently, microRNAs(miRNAs) have been emerged as important regulators in cell differentiation. However, miRNAs which regulate tenocyte differentiation have not been identified. In this study, we used a tenocyte culture system established in our previous study to identify and characterize new miRNAs that control tenocyte differentiation. Our study revealed that miR-675-3p, the expression of which decreases immediately after induction of differentiation, has the effect of promoting differentiation of tenocytes, suppressing the expression of Mef2a and Mef2c detected as its target gene. Our results suggest that miR-675-3p suppresses the expression of Mef2a and Mef2c, whereby controlling tenocyte differentiation.
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