Identification and functional analysis of candidate causal genes of the ossification of posterior longitudinal ligament of the spine

• Project/Area Number: 15K10410
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kagoshima University
• Principal Investigator: Kawamura Ichiro 鹿児島大学, 医歯学総合研究科, 客員准教授 (90535832)
• Co-Investigator(Kenkyū-buntansha): 前田 真吾 鹿児島大学, 医歯学総合研究科, 特任准教授 (60353463) ; 小宮 節郎 鹿児島大学, 医歯学域医学系, 教授 (30178371)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 後縦靭帯骨化症 / GWAS / 脊柱後縦靭帯骨化症 / 骨芽細胞 / 軟骨細胞 / 靭帯細胞 / 軟骨細胞分化 / SnoN / Smpd3

Outline of Final Research Achievements

We tried to isolate mRNA from posterior longitudinal ligament of cervical spine of mice, however, we could not because the tissue was too small. We selected candidate causal genes of OPLL in the sensitive genome area identified by GWAS, and performed immunohistochemistry against the coding proteins. All the selected proteins were detected in the degenerated ligament cells in the OPLL specimens.

Research Products

• [Presentation] 脊柱後縦靭帯骨化症原因候補遺伝子CDC5Lの機能解析 (2017)
  • Author(s): 1.前田真吾, 中島正宏, 河村一郎, 八尋雄平, 冨永博之, 石堂康弘, 武冨栄二, 池川志郎, 小宮節郎
  • Organizer: 第3回日本骨免疫学会
• [Presentation] 後縦靭帯骨化症原因候補遺伝子STK38Lの骨芽細胞分化における機能解析 (2015)
  • Author(s): 前田 真吾
  • Organizer: 厚生労働省科学研究委託費 後縦靭帯骨化症の病態解明・治療法開発に関する研究班ミーティング
  • Place of Presentation: 東京医科歯科大学(東京都文京区)
  • Year and Date: 2015-06-20