Assessment of tumorigenic potential of human induced pluripotent stem cell-derived neural stem/progenitor cells for spinal cord injury
Project/Area Number |
15K10422
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
|
Research Institution | Keio University |
Principal Investigator |
IWANAMI AKIO 慶應義塾大学, 医学部(信濃町), 講師 (40327557)
|
Research Collaborator |
OKANO Hideyuki
NAKAMURA Masaya
KOHYAMA Jun
NISHIMURA Soraya
NISHIYAMA Yuichiro
SUGAI Keiko
IIDA Tsuyoshi
OKUBO Toshiki
Mischel Paul
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 脊髄損傷 / 神経幹細胞 / iPS細胞 / 再生医療 / 腫瘍化 |
Outline of Final Research Achievements |
We have reported the effectiveness of transplanting human iPS cell-derived Neural Stem/Progenitor Cells (hiPS-NS/PCs) for spinal cord injury in rodents as well as primates. Although hiPSC derivatives are considered promising cellular resources for regenerative medicine, their tumorigenicity potentially limits their clinical application. Improved cell quality and safety will be crucially important for any clinical use of hiPSC-NS/PCs. To gain insight into the mechanisms underlying the regulation of tumorigenicity in hiPSC-NS/PCs, we performed a series of integrated DNA methylation and gene expression analyses using tumorigenic and non-tumorigenic hiPSC-NS/PCs. These results indicate that different NS/PC clones have different DNA methylomes and that DNA methylation patterns are unstable as cells are passaged. Therefore, DNA methylation profiles should be included in the criteria used to evaluate the tumorigenicity of hiPSC-NS/PCs in the clinical setting.
|
Report
(4 results)
Research Products
(7 results)
-
[Journal Article] Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells.2017
Author(s)
Itakura G, Ozaki M, Nagoshi N, Kawabata S, Nishiyama Y, Sugai K, Iida T, Kashiwagi R, Ookubo T, Yastake K, Matsubayashi K, Kohyama J, Iwanami A, Matsumoto M, Nakamura M, Okano H.
-
Journal Title
Related Report
Peer Reviewed
-
[Journal Article] Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells.2017
Author(s)
Iida T, Iwanami A, Sanosaka T, Kohyama J, Miyoshi H, Nagoshi N, Kashiwagi R, Toyama Y, Matsumoto M, Nakamura M, Okano H.
-
Journal Title
Stem Cells
Volume: 35
Issue: 5
Pages: 1316-1327
DOI
Related Report
Peer Reviewed
-
[Journal Article] Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives.2017
Author(s)
Itakura G, Kawabata S, Ando M, Nishiyama Y, Sugai K, Ozaki M, Iida T, Ookubo T, Kojima K, Kashiwagi R, Yasutake K, Nakauchi H, Miyoshi H, Nagoshi N, Kohyama J, Iwanami A, Matsumoto M, Nakamura M, Okano H.
-
Journal Title
Stem Cell Reports.
Volume: 8
Issue: 3
Pages: 673-684
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Safe and efficient method for cryopreservation of human induced pluripotent stem cell-derived neural stem and progenitor cells by a programmed freezer with a magnetic field.2016
Author(s)
Nishiyama Y, Iwanami A, Kohyama J, Itakura G, Kawabata S, Sugai K, Nishimura S, Kashiwagi R, Yasutake K, Isoda M, Matsumoto M, Nakamura M, Okano H.
-
Journal Title
Neurosci Res.
Volume: 107
Pages: 20-29
DOI
Related Report
-
[Journal Article] Pretreatment with a γ-Secretase Inhibitor Prevents Tumor-like Overgrowth in Human iPSC-Derived Transplants for Spinal Cord Injury.2016
Author(s)
Okubo T, Iwanami A, Kohyama J, Itakura G, Kawabata S, Nishiyama Y, Sugai K, Ozaki M, Iida T, Matsubayashi K, Matsumoto M, Nakamura M, Okano H.
-
Journal Title
Stem Cell Reports.
Volume: 7
Issue: 4
Pages: 649-663
DOI
Related Report
-
[Journal Article] Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases.2016
Author(s)
Sugai K, Fukuzawa R, Shofuda T, Fukusumi H, Kawabata S, Nishiyama Y, Higuchi Y, Kawai K, Isoda M, Kanematsu D, Hashimoto-Tamaoki T, Kohyama J, Iwanami A, Suemizu H, Ikeda E, Matsumoto M, Kanemura Y, Nakamura M, Okano H.
-
Journal Title
Mol Brain.
Volume: 9
Issue: 1
Pages: 85-85
DOI
Related Report
-