Elucidation of proliferation and metastasis mechanism of platelet-dependent bone soft tissue sarcoma and development of novel therapeutic method

• Project/Area Number: 15K10436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Yamanashi
• Principal Investigator: ICHIKAWA Jiro 山梨大学, 大学院総合研究部, 助教 (00456469)
• Co-Investigator(Renkei-kenkyūsha): INOUE Katsue 山梨大学, 総合研究部, 教授 (10324211) ; HARO Hirotaka 山梨大学, 総合研究部, 教授 (10313264) ; ANDO Takashi 山梨大学, 総合研究部, 講師 (10377492)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨肉腫 / CLEC-2 / 血小板 / ポドプラニン / ＣＬＥＣ－２

Outline of Final Research Achievements

TThe purpose of this study is to elucidate the role of the interaction of osteosarcoma podoplanin and both platelet CLEC-2 interaction in proliferation / metastasis and lead it to therapy. (1)Platelet Aggregation induced by osteosarcoma was confirmed. This aggregation was suppressed with podoplanin antibody and CLEC-2 blocking peptide. (2) Supernatant obtained by coculture with platelet and osteosarcoma induced osteosarcoma migration and EMT partially. (3) Lung metastasis was significantly suppressed when platelet CLEC-2 antibody was administered.

Research Products

• [Presentation] 骨肉腫ポドプラニンによる血小板凝集と活性化 (2017)
  • Author(s): 市川二郎、安藤隆 、佐藤信隆 、大場哲郎 、斉藤正憲 、波呂浩孝
  • Organizer: 第32回日本整形外科学会基礎学術集会