| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Lung metastasis is the major risk factor of osteosarcoma patients. Recently, exosomes have emerged as an important mediator of cell-to-cell signaling through the transfer of molecules such as miRNAs and proteins. Here, we examined the exosomal miRNAs which promote lung metastases of osteosarcoma(OS).
We used the Dunn OS cell line (Dunn) and LM8 OS cell line (LM8) which has highly metastatic potential. More than two times amount of exosome was contained in culture medium of LM8 compared to that of Dunn. LM8 exosomes significantly promoted cell proliferation and migration of Dunn cells. The miRNA array showed the different expression pattern of exosomal miRNA between LM8 and Dunn. The miR-A and miR-B were more included in exosome from LM8 than those of Dunn. The miR-A promoted the proliferation of Dunn, and miR-B promoted the migration of Dunn. The exosomal miR-A and miR-B may play an important role in the formation of lung metastases.
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