Identification and characterization of the exosomal miRNA which promote the progression and metastases of sarcomas
Project/Area Number |
15K10440
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | University of Fukui (2016-2017) Mie University (2015) |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
柿本 拓也 三重大学, 医学系研究科, リサーチアソシエイト (50741162)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 肉腫 / 骨肉腫 / 肺転移 / エクソソーム / マイクロRNA(miRNA) / 遺伝子 / 予後 / 転移 / 再発 / バイオマーカー / 分子標的 |
Outline of Final Research Achievements |
Lung metastasis is the major risk factor of osteosarcoma patients. Recently, exosomes have emerged as an important mediator of cell-to-cell signaling through the transfer of molecules such as miRNAs and proteins. Here, we examined the exosomal miRNAs which promote lung metastases of osteosarcoma(OS). We used the Dunn OS cell line (Dunn) and LM8 OS cell line (LM8) which has highly metastatic potential. More than two times amount of exosome was contained in culture medium of LM8 compared to that of Dunn. LM8 exosomes significantly promoted cell proliferation and migration of Dunn cells. The miRNA array showed the different expression pattern of exosomal miRNA between LM8 and Dunn. The miR-A and miR-B were more included in exosome from LM8 than those of Dunn. The miR-A promoted the proliferation of Dunn, and miR-B promoted the migration of Dunn. The exosomal miR-A and miR-B may play an important role in the formation of lung metastases.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Analysis of Factors for Predicting Survival in Soft-tissue Sarcoma with Metastatic Disease at Initial Presentation2017
Author(s)
Nakamura T, Katagiri H, Shido Y, Hamada S, Yamada K, Nagano A, Yamada S, Tsukushi S, Ishimura D, Matsumine A, Sudo A, Nishida Y
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Journal Title
Anticancer Res
Volume: 37(6)
Pages: 3137-3141
Related Report
Peer Reviewed
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[Journal Article] Phase 2 study of eribulin in patients with previously treated advanced or metastatic soft tissue sarcoma.2017
Author(s)
Kawai A, Araki N, Naito Y, Ozaki T, Sugiura H, Yazawa Y, Morioka H, Matsumine A, Saito K, Asami S, Isu K.
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Journal Title
Jpn J Clin Oncol.
Volume: 47(2)
Issue: 2
Pages: 137-144
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The clinical outcome of pazopanib treatment in Japanese patients with relapsed soft tissue sarcoma: A Japanese Musculoskeletal Oncology Group (JMOG) study.2016
Author(s)
Nakamura T, Matsumine A, Kawai A, Araki N, Goto T, Yonemoto T, Sugiura H, Nishida Y, Hiraga H, Honoki K, Yasuda T, Boku S, Sudo A, Ueda T.
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Journal Title
Cancer
Volume: 122(9)
Issue: 9
Pages: 1408-1416
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Trabectedin monotherapy after standard chemotherapy versus best supportive care in patients with advanced, translocation-related sarcoma: a randomised, open-label, phase 2 study.2015
Author(s)
Kawai A, Araki N, Sugiura H, Ueda T, Yonemoto T, Takahashi M, Morioka H, Hiraga H, Hiruma T, Kunisada T, Matsumine A, Tanase T, Hasegawa T, Takahashi S.
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Journal Title
Lancet Oncol.
Volume: 16
Issue: 4
Pages: 406-16
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Effect of Chronic Hypoxic Environment on Fibrosarcoma Cell Line (HT1080)2016
Author(s)
Iino T, Matsumine A, Matsubara T, Okuno K, Chiba K, Yada Y, Kakimoto T, Nakamura T, Asanuma K, Sudo A
Organizer
ORS 2016 Annual Meeting
Place of Presentation
Disney's coronado springs resort ,Orlando Florida, USA
Year and Date
2016-03-05
Related Report
Int'l Joint Research
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[Presentation] Immunohistochemical Expression and Clinicopathological Assessment of Cancer Testis Antigens (NY-ESO-1, MAGE-A4) in High Grade Soft Tissue Sarcoma2016
Author(s)
Kakimoto T, Matsumine A, Asanuma K, Matsubara T, Nakamura T, Yada Y, Iino T, Sudo A
Organizer
ORS 2016 Annual Meeting
Place of Presentation
Disney's coronado springs resort ,Orlando Florida, USA
Year and Date
2016-03-05
Related Report
Int'l Joint Research
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[Presentation] Biomechanical and Histological Evaluations of Bioactive Pedicle Screws with Hydroxyapatite-Forming Potential in the Canine Lumbar Spine2016
Author(s)
Akeda K, Yamaguchi S, Matsushita T, Kokubo T, Murata K, Takegami N, Yamada J, Matsumine A, Sudo A
Organizer
ORS 2016 Annual Meeting
Place of Presentation
Disney's coronado springs resort ,Orlando Florida, USA
Year and Date
2016-03-05
Related Report
Int'l Joint Research
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[Presentation] Multi-institutional Study of a New Limb Salvage Surgery Using Aciridine Orange in Patients with Bone Sarcomas2016
Author(s)
Matsubara T, Kusuzaki K, Okada K, Tsuchiya T, Goto T, Tsunoda R, Tsuchiya H, Matsumine A, Sudo A
Organizer
AAOS 2016 Annual Meeting
Place of Presentation
Disney's coronado springs resort ,Orlando Florida, USA
Year and Date
2016-03-01
Related Report
Int'l Joint Research
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[Presentation] Effect of Bioactive Pedicle Screws Produced by Chemical and Heat Treatments on Biocompatibility and Bone-bonding Ability: Short-and Middle-term Results2015
Author(s)
Akeda K, Matsushita T, Yamaguchi S, Kokubo T, Murata K, Takegami N, Yamada J, Matsumine A, Uchida A, Sudo A
Organizer
42nd ISSLS Annual Meeting
Place of Presentation
Palace Hotel, SanFransisco ,CA, USA
Year and Date
2015-06-08
Related Report
Int'l Joint Research
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