Novel strategy for bone regeneration using micro RNA

• Project/Area Number: 15K10443
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: Oe Keisuke 神戸大学, 医学部附属病院, 助教 (20514623)
• Co-Investigator(Kenkyū-buntansha): 新倉 隆宏 神戸大学, 医学研究科, 講師 (40448171) ; 李 相亮 昭和大学, 医学部, 講師 (40533732) ; 岩倉 崇 神戸大学, 医学部附属病院, その他 (60437473) ; 福井 友章 神戸大学, 医学部附属病院, 医員 (50437688)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: microRNA / 骨再生 / 骨折 / 偽関節 / 血管新生 / miRNA / 難治性骨折

Outline of Final Research Achievements

After creating a closed femoral fracture model in rats, miRNA was extracted from the newly generated tissue at the fracture site. Microarray and real-time PCR analyses were performed with miRNA samples. From the results, we selected two angionenesis-related miRNAs, miR-126a-3p and miR-146a-5p. We next investigated whether local administration of anti-miRNA oligonucleotides of miR-126a-3p or miR-146a-5p into the fracture site could achieve bone union in a rat refractory femur fracture model. Radilogical and histological assessments revealed that these treatments did not lead to successful bone union at week 8.

Research Products

• [Journal Article] Altered expression of microRNA during fracture healing in diabetic rats. (2018)
  • Author(s): Takahara S, Lee SY, Iwakura T, Oe K, Fukui T, Okumachi E, Waki T, Arakura M, Sakai Y, Nishida K, Kuroda R, Niikura T
  • Journal Title: Bone Joint Res Volume: 7 Issue: 2 Pages: 139-147
  • DOI: 10.1302/2046-3758.72.bjr-2017-0082.r1
  • Peer Reviewed / Open Access
• [Journal Article] Profiling microRNA expression during fracture healing. (2016)
  • Author(s): Waki T, Lee SY, Niikura T, Iwakura T, Dogaki Y, Okumachi E, Oe K, Kuroda R, Kurosaka M.
  • Journal Title: BMC Musculoskelet Disord Volume: 17 Issue: 1 Pages: 83-83
  • DOI: 10.1186/s12891-016-0931-0
  • NAID: 120005714786
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Profiling microRNA expression in fracture nonunions: Potential role of microRNAs in nonunion formation studied in a rat model. (2015)
  • Author(s): Waki T, Lee SY, Niikura T, Iwakura T, Dogaki Y, Okumachi E, Kuroda R, Kurosaka M
  • Journal Title: Bone Joint J. Volume: 97 Issue: 8 Pages: 1144-51
  • DOI: 10.1302/0301-620x.97b8.34966
  • Peer Reviewed
• [Presentation] Analysis of MiRNA Expression Profiles during Fracture Healing in Rats with Diabetes (2018)
  • Author(s): Takahara S, Lee SY, Niikura T, Oe K, Fukui T, Arakura M, Sakai Y, Kuroda R
  • Organizer: Annual meeting of Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] 骨折治癒過程におけるmicroRNAの発現：糖尿病・健常ラット間の比較検討 (2017)
  • Author(s): 高原俊介、李相亮、新倉隆宏、大江啓介、福井友章、新倉路生、黒岩祐、隈部洋平、酒井良忠、黒田良祐
  • Organizer: 日本整形外科学会基礎学術集会
• [Presentation] Altered Expression of MicroRNAs during Fracture Healing in Diabetes Mellitus (2016)
  • Author(s): Takahara S, Lee SY, Niikura T, Iwakura T, Waki T, Arakura M, Sakai Y, Kuroda R, Kurosaka M
  • Organizer: The 62nd Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Orlando, FL, USA
  • Year and Date: 2016-03-05
• [Presentation] 糖尿病ラットの骨折治癒過程におけるmicroRNA発現の解析 (2015)
  • Author(s): 高原 俊介、李 相亮、新倉 隆宏、岩倉 崇、奥町 悦子、脇 貴洋、新倉 路生、酒井 良忠、黒坂 昌弘
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山
  • Year and Date: 2015-10-22