comprehensive study for circadian clock and fracture

• Project/Area Number: 15K10453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Fujiwara Hiroyashi 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (90381962)
• Co-Investigator(Kenkyū-buntansha): 小田 良 京都府立医科大学, 医学(系)研究科(研究院), 講師 (80516469)
• Co-Investigator(Renkei-kenkyūsha): YAGITA KAZUHIRO 京都府立医科大学, 大学院医学研究科・統合生理学教室, 教授 (90324920)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 体内時計 / 骨折修復 / 概日リズム / 発光イメージング / 骨折モデル / 副甲状腺ホルモン / 発行イメージング / PTH / 温度補償性 / 骨折 / 創外固定器

Outline of Final Research Achievements

Circadian clock exists all animal cells, contributes to regulate many physiological functions. We have already unveiled the existence of circadian clock in locomotorium system.But it was not clear how circadian clock function.Thus, we established fracture model and observed what was happened at healing site. Our experimental system prove that circadian clock have also work at fracture site.

Research Products

• [Journal Article] A PTH-responsive circadian clock operates in ex vivo mouse femur fracture healing site (2016)
  • Author(s): Tatsuya Kunimoto, Naoki Okubo, Yoichi Minami, Hiroyoshi Fujiwara, Toshihiro Hosokawa, Maki Asada, Ryo Oda, Toshikazu Kubo, Kazuhiro Yagita
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 22409-22409
  • DOI: 10.1038/srep22409
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Robust Circadian Rhythm and Parathyroid Hormone-Induced Resetting during Hypertrophic Differentiation in ATDC5 Chondroprogenitor Cells (2015)
  • Author(s): Toshihiro Hosokawa, Yoshiki Tsuchiya, Naoki Okubo, Tatsuya Kunimoto, Yoichi Minami, Hiroyoshi Fujiwara, Yasuhiro Umemura, Nobuya Koike, Toshikazu Kubo, Kazuhiro Yagita
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 48 Issue: 6 Pages: 165-171
  • DOI: 10.1267/ahc.15025
  • NAID: 130005116781
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Open Access
• [Presentation] Visualization of the circadian clock in skeletal bones obtained from neonatal Per2::Luc mice using bioluminescence macro-imaging device (2017)
  • Author(s): Tatsuya Kunimoto, Yoichi Minami, Naoki Okubo, Hiroyoshi Fujiwara, Toshihiro Hosokawa, Ryo Oda, Toshikazu Kubo, Kazuhiro Yagita
  • Organizer: 63th Annual meeting of Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] Phase shift of the circadian clock by heat stimulation in MC3T3-E1 (2017)
  • Author(s): Toshihiro Hosokawa, Yoshiki Tsuchiya, Naoki Okubo, Tatsuya Kunimoto, Yoichi Minami, Hiroyoshi Fujiwara, Ryo Oda, Toshikazu Kubo, Kazuhiro Yagita
  • Organizer: 63th Annual meeting of Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] Visualization of the circadian clock in skeletal bones obtained from neonatal Per2::Luc mice using bioluminescence macro-imaging device, ORS 2017 Annual Meeting (2017)
  • Author(s): Tatsuya Kunimoto, Yoichi Minami, Naoki Okubo, Hiroyoshi Fujiwara, Toshihiro Hosokawa, Ryo Oda, Toshikazu Kubo, Kazuhiro Yagita
  • Organizer: 63th Annual meeting of Orthopaedic Research Society
  • Place of Presentation: San Diego, the U.S.
  • Int'l Joint Research
• [Presentation] Parathyroid hormone-induced resetting of circadian clock in ATDC5 (2016)
  • Author(s): Toshihiro Hosokawa, Yoshiki Tsuchiya, Naoki Okubo, Tatsuya Kunimoto, Yoichi Minami, Hiroyoshi Fujiwara, Ryo Oda, Toshihiro Kubo, Kazuhiro Yagita
  • Organizer: 62th Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Orland, United States
  • Year and Date: 2016-03-06
• [Presentation] Temperature is a time cue to cartilages (2016)
  • Author(s): Naoki Okubo, Yoichi Minami, Hiroyoshi Fujiwara, Tatsuya Kunimoto, Toshihiro Hosokawa, Ryo Oda, Toshihiro Kubo, Kazuhiro Yagita
  • Organizer: 62th Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Orland, United States
  • Year and Date: 2016-03-06
• [Presentation] Parathyroid hormone entrainable circadian clock functions in the mouse femur fracture healing site (2016)
  • Author(s): Tatsuya Kunimoto, Naoki Okubo, Yoichi Minami, Hiroyoshi Fujiwara, Toshihiro Hosokawa, Ryo Oda, Toshihiro Kubo, Kazuhiro Yagita
  • Organizer: 62th Annual Meeting of Orthopaedic Research Society
  • Place of Presentation: Orland, United States
  • Year and Date: 2016-03-05
• [Presentation] マウス大腿骨器官培養系を用いた治癒過程の骨折部における体内時計の観察，第31回日本整形外科学会基礎学術集会 (2016)
  • Author(s): 國本達哉，大久保直輝，南 陽一，藤原浩芳，細川俊浩，小田 良，久保俊一，八木田和弘
  • Organizer: 第31回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡
• [Presentation] 骨芽細胞における体内時計と温熱刺激の関係 (2016)
  • Author(s): 細川俊浩，土谷佳樹，國本達哉，大久保直輝，南 陽一，藤原浩芳，小田 良，久保俊一，八木田和弘
  • Organizer: 第31回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡
• [Presentation] 軟骨部体内時計における温熱刺激の影響 (2015)
  • Author(s): 大久保直輝，藤原浩芳，南 陽一，國本達哉，細川俊浩，小田 良，久保俊一，八木田和弘
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2015-10-22
• [Presentation] PTH（1－34）による治癒過程の骨折部における体内時計の位相変位 (2015)
  • Author(s): 國本達哉，大久保直輝，南 陽一，藤原浩芳，細川俊浩，小田 良，久保俊一, 八木田和弘
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2015-10-22
• [Presentation] PTH(1-34)による軟骨前駆細胞株ATDC5の分化依存的な概日リズムの同調作用 (2015)
  • Author(s): 細川俊浩, 土谷佳樹, 大久保直輝, 國本達哉, 南 陽一, 藤原浩芳, 小田 良, 久保俊一, 八木田和弘
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2015-10-22