Development of a suspended synovium culture model mimicking inside of the knee joint

• Project/Area Number: 15K10463
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KATANO Hisako 東京医科歯科大学, 統合研究機構, 助教 (50376620)
• Co-Investigator(Kenkyū-buntansha): 関矢 一郎 東京医科歯科大学, 統合研究機構, 教授 (10345291)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 再生医療 / 再生医学

Outline of Final Research Achievements

Drugs that induce mesenchymal stem cells (MSCs) from the synovium can be a promising treatment for mild tissue injury. We developed a suspended synovium culture model. Fibrous synovium was found to release greater numbers of MSCs than adipose synovium. Mobilization of MSCs from the synovium was demonstrated using a suspended synovium culture model in RA. This model can be a valuable tool to screen drugs which induce mobilization of MSCs from the synovium into synovial fluid in the knee joint.

Research Products

• [Journal Article] Comparison of mesenchymal stem cells obtained by suspended culture of synovium from patients with rheumatoid arthritis and osteoarthritis. (2018)
  • Author(s): Yuji Kohno, Mitsuru Mizuno, Nobutake Ozeki, Hisako Katano, Koji Otabe, Hideyuki Koga, Mikio Matsumoto, Haruka Kaneko, Yuji Takazawa, Ichiro Sekiya
  • Journal Title: BMC Musculoskelet Disord Volume: 19 Issue: 1 Pages: 78-78
  • DOI: 10.1186/s12891-018-1998-6
  • Peer Reviewed / Open Access
• [Journal Article] Fibrous synovium releases higher numbers of mesenchymal stem cells than adipose synovium in a suspended synovium culture model. (2017)
  • Author(s): Kenta Katagiri, Yu Matsukura, Takeshi Muneta, Nobutake Ozeki, Mitsuru Mizuno, Hisako Katano, Ichiro Sekiya
  • Journal Title: Arthroscopy Volume: 33 Issue: 4 Pages: 800-810
  • DOI: 10.1016/j.arthro.2016.09.033
  • Peer Reviewed / Open Access
• [Presentation] Harvested cell number varies greater in RA than in OA after a suspended synovium culture (2017)
  • Author(s): Yuji Kohno, Mitsuru Mizuno, Kenta Katagiri, Koji Otabe, Nobutake Ozeki, Hisako Katano, Keiichiro Komori, Masafumi Horie, Kunikazu Tsuji, Mikio Matsumoto, Haruka Kaneko, Yuji Takazawa, Takeshi Muneta, Ichiro Sekiya
  • Organizer: Orthopaedic Research Society (ORS) 2017 Annual Meeting
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2017-03-21
  • Int'l Joint Research
• [Presentation] 浮遊滑膜培養モデルにおける関節リウマチ膝および変形性関節症膝の滑膜幹細胞 (2017)
  • Author(s): 河野 佑二、水野 満、片桐 健太、小田邉 浩二、大関 信武、片野 尚子、小森 啓一郎、 堀江 雅史、辻 邦和、松本 幹生、金子 晴香、高澤 祐治、宗田 大、関矢 一郎
  • Organizer: 第30回日本軟骨代謝学会
  • Place of Presentation: みやこメッセ（京都市）
  • Year and Date: 2017-03-04
• [Presentation] Fibrous Synovium Releases Higher Number Of MSCs Than Adipose Synovium in a Suspended Synovium Culture Model (2016)
  • Author(s): Kenta Katagiri, Takeshi Muneta, Koji Otabe, Yu Matsukura, Hisako Katano,Ichiro Sekiya
  • Organizer: Orthopaedic Research Society 2016 Annual Meeting
  • Place of Presentation: Florida（米国）
  • Year and Date: 2016-03-05
  • Int'l Joint Research
• [Presentation] 線維性滑膜は脂肪性滑膜よりも多くの間葉系幹細胞を動員する：浮遊滑膜モデルによる解析 (2016)
  • Author(s): 片桐健太、松倉 遊、宗田 大、大関信武、水野 満、小田邉浩二、片野尚子、古賀英之、辻 邦和、関矢一郎
  • Organizer: 第29回日本軟骨代謝学会
  • Place of Presentation: 広仁会館（広島県、広島市）
  • Year and Date: 2016-02-19
• [Presentation] 浮遊滑膜産生細胞培養モデルによる解析 (2015)
  • Author(s): 片桐 健太, 宗田 大, 松倉 遊, 辻 邦和, 大川 淳, 関矢 一郎
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山国際会議場（富山県、富山市）
  • Year and Date: 2015-10-22