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Periodic injections of adipose-derived stem cell sheets inhibit osteoarthritis progression in an experimental rabbit model

Research Project

Project/Area Number 15K10465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKanazawa University

Principal Investigator

Kabata Tamon  金沢大学, 附属病院, 准教授 (10334749)

Co-Investigator(Kenkyū-buntansha) 林 克洋  金沢大学, 大学病院, 助教 (80507054)
高木 知治  金沢大学, 医学系, 協力研究員 (40749173)
方 向  金沢大学, 医学系, 博士研究員 (20750350)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords脂肪由来幹細胞 / セルシート技術 / 変形性膝関節症 / 再生医療 / 脂肪幹細胞 / paracrine effect
Outline of Final Research Achievements

Adipose-derived stem cells (ADSCs) can easily be isolated from subcutaneous adipose tissue and cultured in large quantities with a minimally invasive procedure. So, our study team has investigated them in various fields such as bones, cartilages, peripheral nerves, menisci and ligaments. The purpose of this study was to determine whether intra-articularly injected ADSC sheets inhibited articular cartilage degeneration during osteoarthritis(OA) development in a rabbit anterior cruciate ligament transection (ACLT) model.
Our study showed that the OA progression was milder in the ADSC sheets group. Furthermore, collagenases (MMP-1, MMP-13) and aggrecanase (ADAMTS-4) were less expressive in the same group. So, we suggested that the ADSCs inhibited progression of articular cartilage degeneration by secreting liquid factors having chondro-protective effects. To the best of our knowledge, our study is the first to prove the role of ADSC sheets in the treatment of OA.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report

Research Products

(20 results)

All 2018 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Effectiveness and Safety of Needle Medial Collateral Ligament Pie-Crusting in Total Knee Arthroplasty: A Cadaveric Study.2018

    • Author(s)
      Ohmori T, Kabata T, Kajino Y, Takagi T, Tsuchiya H.
    • Journal Title

      J Knee Surg

      Volume: 5 Pages: 1-1

    • DOI

      10.1055/s-0037-1606377

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CS型TKAにおいてインサートが前後安定性に与える影響-KS measure(十字靭帯機能検査機器)を用いた屍体研究-2017

    • Author(s)
      高木 知治,前田 亨,大森隆昭,土屋 弘行
    • Journal Title

      JOSKAS

      Volume: 42 Pages: 264-265

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 変形性股関節症に対するエコーガイド下関節内ステロイド投与の臨床効果2017

    • Author(s)
      高木知治,加畑多文,楫野良知,井上大輔,山本崇史,大森隆昭,土屋弘行
    • Journal Title

      Hip Joint

      Volume: 43 Pages: 995-997

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The correlation between clinical radiological outcome and contact state of implant and femur using three-dimensional templating software in cementless total hip arthroplasty.2016

    • Author(s)
      Inoue D, Kabata T, Maeda T, Kajino Y, Yamamoto T, Takagi T, Ohmori T, Tsuchiya H.
    • Journal Title

      Eur J Orthop Surg Traumatol.

      Volume: 26 Pages: 591-598

    • DOI

      10.1007/s00590-016-1796-5

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ARMD症例に対するdual mobility systemを用いた再置換術の経験.2016

    • Author(s)
      高木 知治,加畑 多文,前田 亨,楫野 良知,井上 大輔,山本 崇史,大森 隆昭,土屋 弘行
    • Journal Title

      Hip Joint

      Volume: 42 Pages: 581-4

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] ポリオ麻痺後のlong leg arthropathyに対してrotating hinge型TKAを施行した一例.2016

    • Author(s)
      高木 知治,前田 亨,加畑 多文,楫野 良知,井上 大輔,土屋 弘行
    • Journal Title

      中部整災誌

      Volume: 59 Pages: 313-4

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.2015

    • Author(s)
      Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H.
    • Journal Title

      BMC Musculoskelet Disord

      Volume: 16

    • DOI

      10.1186/s12891-015-0701-4

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Iodine-Supported Hip Implants: Short Term Clinical Results.2015

    • Author(s)
      Kabata T, Maeda T, Kajino Y, Hasegawa K, Inoue D, Yamamoto T, Takagi T, Ohmori T, Tsuchiya H.
    • Journal Title

      Biomed Res Int.

      Volume: 2015

    • DOI

      10.1155/2015/368124

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] PCLを温存したCS型TKAの動作解析-イメージフリーナビゲーションシステムを用いた屍体研究2015

    • Author(s)
      高木知治,前田亨,加畑多文,楫野良知,長谷川和宏,井上大輔,山本崇史,土屋弘行
    • Journal Title

      日本人工関節学会誌

      Volume: 45 Pages: 505-506

    • Related Report
      2015 Research-status Report
  • [Presentation] Periodic Injections Of Adipose-Derived Stem Cells Sheet Inhibit Osteoarthritis Progression In An Experimental Rabbit Model.2018

    • Author(s)
      Takagi T, Kabata T, Kajino Y, Taga T, Hasegawa K, Inoue D, Yamamoto T, Ohmori T, Yoshitani J, Ueno T, Tsuchiya H
    • Organizer
      AAOS 2018 annual meeting
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂肪由来幹細胞シートによる軟骨保護効果-家兎変形性膝関節症モデルにおける検討-2017

    • Author(s)
      高木 知治,加畑 多文,林 克洋,楫野 良知,多賀 正,井上 大輔,山本 崇史,大森 隆昭,吉谷 純哉,上野 琢郎,土屋 弘行
    • Organizer
      第32回 日本整形外科学会基礎学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Periodic Injections Of Adipose-Derived Stem Cells Sheet Inhibit Osteoarthritis Progression In An Experimental Rabbit Model.2017

    • Author(s)
      Takagi T, Kabata T, Kajino Y, Taga T, Hasegawa K, Inoue D, Yamamoto T, Ohmori T, Yoshitani J, Ueno T, Tsuchiya H
    • Organizer
      2017 European Federation of National Associations of Orthopaedics and Traumatology (EFORT)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CS型TKAにおいてインサートが前後安定性に与える影響2016

    • Author(s)
      高木 知治,前田 亨,加畑 多文,楫野 良知,井上 大輔,山本 崇史,大森 隆昭,土屋 弘行
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2016-10-13
    • Related Report
      2016 Research-status Report
  • [Presentation] Motion Analysis after Cruciate-Substituting Total Knee Arthroplasty Suggests the Role of Posterior Cruciate Ligament: A Cadaveric Study.2016

    • Author(s)
      Takagi T, Maeda T, Kabata T, Kajino Y, Inoue D, Yamamoto T, Ohmori T, Tsuchiya H
    • Organizer
      The 16th Annual Meeting of the International Society for Computer Assisted Orthopaedic Surgery (CAOS International)
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2016-06-09
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Optimal posterior reference point for sizing of the femoral component in total knee arthroplasty.2016

    • Author(s)
      Takagi T, Maeda T, Kabata T, Kajino Y, Inoue D, Yamamoto T, Ohmori T, Tsuchiya H
    • Organizer
      2016 European Federation of National Associations of Orthopaedics and Traumatology (EFORT)
    • Place of Presentation
      Geneva, Switzerland
    • Year and Date
      2016-06-01
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] PCL の有無がCS 型TKA のkinematics に与える影響 ‐イメージフリーナビゲーションシステムを用いた屍体研究‐2016

    • Author(s)
      高木 知治,前田 亨,加畑 多文,楫野 良知,井上 大輔,山本 崇史,大森 隆昭,土屋 弘行
    • Organizer
      日本整形外科学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2016-05-12
    • Related Report
      2016 Research-status Report
  • [Presentation] ヨード担持表面処理を施したチタン製インプラントの実用化への試み2015

    • Author(s)
      加畑多文, 楫野良知, 白井寿治, 土屋弘行
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      富山国際会議場(富山県富山市)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Research-status Report
  • [Presentation] CS型TKAにおいてPCLの有無がkinematicsに与える影響 ―イメージフリーナビゲーションシステムを用いた屍体研究―2015

    • Author(s)
      高木知治, 前田亨, 加畑多文, 楫野良知, 長谷川和宏, 井上大輔, 山本崇史, 土屋弘行
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      富山国際会議場(富山県富山市)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Early Dislocation Rate In Computer Navigation Assisted Primary Total Hip Arthroplasty2015

    • Author(s)
      Kabata T, Maeda T, Kajino Y, Kuroda K, Hasegawa K, Inoue D, Yamamoto T, Takagi T, Tsuchiya H
    • Organizer
      16th European Federation of National Associations of Orthopaedics and Traumatology
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2015-05-27
    • Related Report
      2015 Research-status Report
  • [Presentation] Motion Analysis After Cruciate-Substituting Total Knee Arthroplasty Suggests The Role Of Posterior Cruciate Ligament: A Cadaveric Study2015

    • Author(s)
      Takagi T, Toru M, Kabata T, Kajino Y, Hasegawa K, Inoue D, Yamamoto T, Tsuchiya H
    • Organizer
      16th European Federation of National Associations of Orthopaedics and Traumatology
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2015-05-27
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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