| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
P21 was identified as cell cycle regulator which its induction by p53 during a DNA damage-induced G (1)-phase checkpoint response inhibits both Cyclin-dependent kinase activities.We evaluated synovitis with H-E stain section. At postoperative 1week, both p21(-/-) and WT showed synovial membrane outgrowth. At 8week, synovial inflammation was postponed only in p21(-/-) mice.
Macrophage invasion to synovium was analyzed with F4/80 immunohistochemistry showed that. surface linier of synovial membranes were strongly stained in p21(-/-) mice compared with WT at1 or 8week. We evaluated and found that synovial membrane of p21(-/-) mice were strongly stained compared with Wt. Safranin O staining in joint cartilage tissues were evaluated by OARSI histopathology classification. P21(-/-) mice post DMM surgery at 8 weeks showed severe cartilage degeneration.
We demonstrated that the p21 deficiency was susceptible to osteoarthritis change with macrophage infiltration to synovial tissues.
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