The influence of gelatin hydrogel including eicosapentaenoic acid on the osteoarthritis progression in vivo.

• Project/Area Number: 15K10472
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: Hayashi Shinya 神戸大学, 医学部附属病院, 助教 (20437487)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: EPA / 軟骨 / 変形性関節症 / 炎症

Outline of Final Research Achievements

Eicosapentaenoic acid (EPA) is an antioxidant and omega-3 (n-3) polyunsaturated fatty acid that reduces the production of inflammatory cytokines. We previously reported that EPA treatment inhibited chondrocyte apoptosis and MMP expression induced by oxidative stress in vitro, and prevented osteoarthtitis (OA) progression in vivo.The purpose of this study is to investigate the effect of gelatin hydrogel including EPA on the OA progression in vivo.Thirty-six male C57BL/6J mice were randomly divided into 6 groups (n = 6 mice each): sham, DMM, DMM injected with corn oil, DMM injected with corn oil and EPA, DMM with control gelatin hydrogel and DMM with gelatin hydrogel including EPA. Corn oil or corn oil with EPA was injected once into the knee joint at timing of DMM surgery.we investigated the effect of gelatin hydrogel including EPA on the OA progression in vivo. The gelatin hydrogel including EPA prevented OA progression more effective than single injection of EPA in vivo.

Research Products

• [Presentation] The influence of gelatin hydrogel including eicosapentaenoic acid on the osteoarthritis progression in vivo. (2018)
  • Author(s): Tsubosaka M, Hayashi S, Kihara S, Nagata J, Kuroda Y, Takeuchi K, Haneda M, Kirizuki S, Takayama K, Hashimoto S, Matsumoto T, Tabata Y, Kuroda R.
  • Organizer: The 64th Annual Meeting, Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] エイコサペンタエン酸は酸化ストレス依存性の軟骨細胞の変性、アポトーシスを抑制する (2016)
  • Author(s): 木原伸介、林申也、明石祐典、永田純平、神崎至幸、橋本慎吾、鎮西伸顕、羽田勝彦、竹内一裕、黒田良祐、田畑泰彦、黒坂昌弘
  • Organizer: 第31回日本整形外科学会基礎学術集会
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2016-10-13