Establishment of rapidly testing bacterial gene using PCR lateral flow assay in periprosthetic joint infection
| Project/Area Number |
15K10489
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kitasato University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
SAKURAI KEIZOU 北里大学病院, 臨床検査部, 主任
NIHONYANAGI SHIN 北里大学病院, 臨床検査部・感染管理室
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 人工関節周囲感染 / PCRラテラルフロー法 / 迅速細菌遺伝子検査 / シーケンス解析 / 16S-rRNA / MRSA / αディフェンシン / 人工関節 / 感染 |
|---|
| Outline of Final Research Achievements |
The gold standard for diagnosing periprosthetic joint infections (PJI) is bacterial culture. Unfortunately, it usually takes a few days for the results of these cultures to be obtained. In this study, the objective was to determine whether PCR-lateralflow (PCR-LF) tests could be used to effectively diagnose PJI. We used PCR-LF to diagnose common causes of PJI and compared the results to those from conventional bacterial cultures of the same samples. Two genetic markers specific to MRSA were being detected during this analysis. PCR results were interpreted as follows MRSA, MSSA, MR-CNS or negative. Our data indicate that PCR-LF was superior to bacterial culture tests in terms of sensitivity, particularly for PJI samples. In conclusion, our results showed that PCR-LF is a useful tool for identifying pathogenic bacteria in PJI.
|
Report
(4 results)
Research Products
(15 results)
-
-
-
-
-
[Presentation] Clinical evaluation of efficacy using PCR lateral-flow assay for Gram-positive and Gram-negative bacterial infectiondetection in joint fluid2017
Author(s)
Katsufumi Uchiyama, Hiromi Kanda, Tokiko Yamaguchi, Shiro Ibe, Keizou Sakurai, Shin Nihonyanagi, Yojiro Minegishi, Shinsuke Ikeda, Yusaku Kanou,Kensuke Fukushima,Naonobu Takahira, Masashi Takaso
Organizer
36th Annual meeting of the European Bone and Joint Infection Society
Related Report
Int'l Joint Research
-
-
-
[Presentation] 人工関節周囲感染の予防と早期診断の試み2016
Author(s)
内山勝文,峯岸洋次郎,福島健介,森谷光俊,山本豪明,池田信介,高相晶士,山本豪明,高平尚伸
Organizer
第43回日本股関節学会学術集会
Place of Presentation
大阪国際会議場（大阪府大阪市）
Year and Date
2016-11-04
Related Report
-
-
[Presentation] 人工股関節周囲感染に対するステムを温存した治療法の工夫2016
Author(s)
内山勝文,森谷光俊,福島健介,山本豪明,峯岸洋次郎, 田信介,高相晶士,山本豪明,峯岸洋次郎,池田信介,高平尚伸
Organizer
第39回日本骨・関節感染症学会
Place of Presentation
岡山コンベンションセンター(岡山県岡山市）
Year and Date
2016-07-08
Related Report
-
-
-
-
-
