| Project/Area Number |
15K10501
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
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Principal Investigator |
Miyazaki Tsuyoshi 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (50376480)
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| Co-Investigator(Kenkyū-buntansha) |
時村 文秋 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (80242147)
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| Co-Investigator(Renkei-kenkyūsha) |
SAWADA YASUHIRO 国立障害者リハビリテーションセンター(研究所), 研究所 運動機能系障害研究部, 部長 (50313135)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | メカニカルストレス / 骨細胞 / unloading / p130Cas |
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| Outline of Final Research Achievements |
Osteocytes are recognized as mechanosensory cells, although it is largely unknown how bone metabolism is mechanically modulated. Here, we report that p130Cas (Cas) plays a major role in osteocyte mechanosensing to subsequently support bone homeostasis. Osteocyte-specific Cas knockout (Cas cKO) mice exhibit osteopenia. We used a mouse bone-unloading model, in which one of the hindlimbs of each individual mouse was subjected to sciatic and femoral nerve resection. Unloaded femurs exhibited a significant reduction of bone volume as compared with their contralateral controls. Unloaded bones of Casflox/flox mice exhibited significant reduction of bone volume with significantly increased bone resorption parameters. In contrast, bone resorption parameters remained unaltered upon unloading in Cas cKO mice, although they showed a tendency of unloading-induced decrease in bone volume. These results suggest that Cas in osteocytes is involved in the mechanosensory function in bone.
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