| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We investigated the protein binding site of lidocaine to elucidate the target of the lidocaine toxicity. Lidocaine and FG beads were immobilized for using as probe of lidocaine binding protein. Lysate of Hela cells was mixed with the probe and the fractions were corrected to western blotting for detection of the receptor. Several bands were detected at 40 kDa, 50 kDa, and 60 kDa in the wester blotting. However, affinity of the lidocaine binding probe was very low and the specific binding could not be detected.
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