| Project/Area Number |
15K10520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
竹中 重雄 大阪府立大学, 総合リハビリテーション学研究科, 教授 (10280067)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脳神経疾患 / 脳虚血 / 海馬 / 虚血耐性 / 海馬神経細胞 / アコニターゼ / アルドラーゼ |
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| Outline of Final Research Achievements |
This study aimed to examine whether aconitase 2, an enzyme of tricarboxylic acid cycle, and aldolase A, an enzyme of glycolytic system, are key proteins for protecting neurons from ischemic injury using a cultured fetal hippocampal neurons. In this study, in vitro model of ischemia were mimicked by oxygen-glucose deprivation (OGD). OGD-induced cell death was assessed by measuring the activity of lactate dehydrogenase (LDH) that leaked into the culture medium. OGD for 180 min induced significantly neuronal cell death. The decrease in expression level of aconitase 2 mRNA and protein after ransfection of aconitase 2 siRNA were confirmed by RT-PCR and western blot, respectively. There was not significant difference in OGD-induced neuronal cell death between aconitase 2 siRNA transfected neurons and control siRNA transfected neurons.
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