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In vitro study of the functinal significane of ischemic tolerance-induced changes in the expression level of aconitase2 and aldolase A

Research Project

Project/Area Number 15K10520
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Anesthesiology
Research InstitutionOsaka Prefecture University

Principal Investigator

Nakajima Takayuki  大阪府立大学, 生命環境科学研究科, 准教授 (30333644)

Co-Investigator(Kenkyū-buntansha) 竹中 重雄  大阪府立大学, 総合リハビリテーション学研究科, 教授 (10280067)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords脳神経疾患 / 脳虚血 / 海馬 / 虚血耐性 / 海馬神経細胞 / アコニターゼ / アルドラーゼ
Outline of Final Research Achievements

This study aimed to examine whether aconitase 2, an enzyme of tricarboxylic acid cycle, and aldolase A, an enzyme of glycolytic system, are key proteins for protecting neurons from ischemic injury using a cultured fetal hippocampal neurons. In this study, in vitro model of ischemia were mimicked by oxygen-glucose deprivation (OGD). OGD-induced cell death was assessed by measuring the activity of lactate dehydrogenase (LDH) that leaked into the culture medium. OGD for 180 min induced significantly neuronal cell death. The decrease in expression level of aconitase 2 mRNA and protein after ransfection of aconitase 2 siRNA were confirmed by RT-PCR and western blot, respectively. There was not significant difference in OGD-induced neuronal cell death between aconitase 2 siRNA transfected neurons and control siRNA transfected neurons.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2017 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Proteomic analysis of the hippocampus in naive and ischemic-preconditioned rat2015

    • Author(s)
      Nakajima T, Hata R, Kondo T, Takenaka S
    • Journal Title

      Journal of Neurological Science

      Volume: 358 Issue: 1-2 Pages: 158-171

    • DOI

      10.1016/j.jns.2015.08.1530

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 虚血性プレコンディショニング後のラット海馬CA1領域におけるタンパク質発現の変化2017

    • Author(s)
      中島崇行、竹中重雄
    • Organizer
      日本病態生理学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 全脳虚血後の海馬におけるSmad免疫陽性のグリア細胞の動態について2017

    • Author(s)
      国枝祐志、中島崇行、近藤友宏
    • Organizer
      日本獣医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 虚血性プレコンディショニング後のラット海馬におけるタンパク質発現の変化2015

    • Author(s)
      中島崇行、竹中重雄
    • Organizer
      第158回日本獣医学会
    • Place of Presentation
      北里大学獣医学部(青森県十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Research-status Report
  • [Presentation] ラット海馬CA1領域とCA3/DG領域間で発現レベルが異なるタンパク質のプロテオーム解析による探索2015

    • Author(s)
      中島崇行、竹中重雄
    • Organizer
      第25回日本病態生理学会
    • Place of Presentation
      愛媛大学(愛媛県松山市)
    • Year and Date
      2015-07-31
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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