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Molecular biological study on action mechanism of general anesthetics

Research Project

Project/Area Number 15K10525
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Anesthesiology
Research InstitutionNippon Medical School

Principal Investigator

Sakamoto Atsuhiro  日本医科大学, 大学院医学研究科, 大学院教授 (30196084)

Research Collaborator 森田 智教  日本医科大学, 大学院生
大槻 達郎  日本医科大学, 大学院生
木村 祐輔  日本医科大学, 大学院生
藤本 彩子  日本医科大学, 大学院生
松尾 いずみ  日本医科大学, 大学院生
永本 盛嗣  日本医科大学, 大学院生
岩崎 雅江  日本医科大学, 大学院生
伊藤 直美  日本医科大学, 大学院生
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords全身麻酔薬 / 遺伝子発現 / microRNA / 虚血再潅流障害 / 全身炎症性反応 / 神経発達 / 日内変動 / 虚血再潅流 / micro RNA / 麻酔 / 全身性炎症反応 / 発達形成
Outline of Final Research Achievements

The effects of general anesthetics themselves and the effects of general anesthetics at various disease states on the changes of internal gene expression, especially microRNA (miRNA) expression were evaluated. How these effects are related to gene expression (messenger RNA: mRNA), protein expression and metabolites affected by general anesthesia were also evaluated. The results obtained from examinations of several conditions such as anesthetic exposure on newborn rats, ischemia-reperfusion injury, systemic inflammatory response syndrome, provided the basic data of miRNA expression for the mechanism elucidation of the general anesthesia.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 7 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] miR-15b mediates oxaliplatin-induced chronic neuropathic pain through BACE1 down-regulation.2017

    • Author(s)
      Ito N, Sakai A, Miyake N, Maruyama M, Iwasaki H, Miyake K, Okada T, Sakamoto A, Suzuki H.
    • Journal Title

      British Journal of Pharmacology

      Volume: 174 Issue: 5 Pages: 386-395

    • DOI

      10.1111/bph.13698

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] miR-15b mediates oxaliplatin-induced chronic neuropathic pain through BACE1 down-regulation.2017

    • Author(s)
      to N, Sakai A, Miyake N, Maruyama M, Iwasaki H, Miyake K, Okada T, Sakamoto A, Suzuki H.
    • Journal Title

      Br J Pharmacol.

      Volume: 174 Pages: 386-395

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of sevoflurane effects on Per2 expression using exvivo bioluminescence imaging of the suprachiasmatic nucleus in transgenic rats2016

    • Author(s)
      Matsuo I.,Iijima N.,Takumi K.,Higo S.,Aikawa S.,Anzai M., Ishii H., Sakamoto A.,Ozawa H
    • Journal Title

      Neuroscience Research

      Volume: in press Pages: 30-37

    • DOI

      10.1016/j.neures.2015.11.010

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Volatile anaesthetics enhance the metastasis related cellular signalling including CXCR2 of ovarian cancer cells.2016

    • Author(s)
      Iwasaki M, Zhao H, Jaffer T, Unwith S, Benzonana L, Lian Q, Sakamoto A, Ma D
    • Journal Title

      Oncotarget

      Volume: 7 Pages: 26042-26056

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Establishment of an in vitro cell line experimental system for the studablishment of an in vitro cell line experimental system for the study oy of inhalational anesthetic mechanisms.2016

    • Author(s)
      Nagamoto S, Iijima N, Ishii H, Takumi K, Higo S, Aikawa S, Anzai M, Matsuo I, Nakagawa S, Takashima N, Shigeyoshi Y, Sakamoto A, Ozawa H.
    • Journal Title

      Neurosci Lett.

      Volume: 4;620: Pages: 163-168

    • DOI

      10.1016/j.neulet.2016.04.005

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identical microRNAs regulate liver protection during anesthetic and ischemic preconditioning in rats: animal study2015

    • Author(s)
      Morita T, Ishikawa M, Sakamoto A
    • Journal Title

      PLoS One

      Volume: 10 Issue: 5 Pages: e0125866-e0125866

    • DOI

      10.1371/journal.pone.0125866

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Volatile anesthetic sevoflurane ameliorates endotoxin-induced acute lung injury via microRNA modulation in rats2015

    • Author(s)
      Otsuki T, Ishikawa M, Hori Y, Goto G, Sakamoto A
    • Journal Title

      Biomedical Reports

      Volume: 3 Pages: 408-412

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of electroconvulsive stimulation on messenger RNA expression in the prefrontal cortes in a rat pain model2015

    • Author(s)
      Kimura Y, Ishikawa M, Hori Y, Okabe T, Sakamoto A
    • Journal Title

      Biomedical Reports

      Volume: 3 Pages: 802-806

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] <b>Influence of neonatal sevoflurane exposure on nerve development-related microRNAs and behavior of </b><b>rats </b>2015

    • Author(s)
      Fujimoto Y, Ishikawa M, Nagano M, Sakamoto A
    • Journal Title

      Biomedical Research

      Volume: 36 Issue: 6 Pages: 347-355

    • DOI

      10.2220/biomedres.36.347

    • NAID

      130005116839

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 吸入麻酔薬作用機構の研究のためのcell lineを用いたin vivtro実験系の確立2016

    • Author(s)
      永本盛嗣,坂本篤裕
    • Organizer
      第63回日本麻酔学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2016-05-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 一次感覚神経節miR-15bのオキサリプラチン誘発性神経障害疼痛における解析2016

    • Author(s)
      黒木直美,坂本篤裕
    • Organizer
      第63回日本麻酔学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2016-05-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Influence of neonatal sevoflurane exposure on nerve development-related microRNAs and behavior of rats2015

    • Author(s)
      Fujimoto S, Nagano M, Ishikawa M, Sakamoto A
    • Organizer
      American Society of Anesthesiologists. Annual meeting 2015
    • Place of Presentation
      San Diego, California, USA
    • Year and Date
      2015-10-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 神経因性障害モデルラットに対するElectroconvulsive stimulationによる前頭野のmessengerRNA変化の検討2015

    • Author(s)
      木村祐輔、保利陽子、石川真士、坂本篤裕
    • Organizer
      第62回日本麻酔科学会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] オキサリプラチン誘発性神経障害性疼痛における後根神経節でのマイクロRNAの発現変化2015

    • Author(s)
      伊藤直美、坂井敦、鈴木秀典、坂本篤裕
    • Organizer
      第62回日本麻酔科学会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] ラット幼若期セボフルラン暴露が発達段階の海馬microRNA発現変化へ及ぼす影響2015

    • Author(s)
      藤本彩子、永野昌俊、石川真士、坂本篤裕
    • Organizer
      第62回日本麻酔科学会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 肝臓虚血再灌流モデルにおけるセボフルランプレコンディショニングと虚血プレコンディショニングのmicroRNA発現変化の比較2015

    • Author(s)
      森田智教、石川真士、坂本篤裕
    • Organizer
      第62回日本麻酔科学会学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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