Clinical use of functional regulation of aquaporin for brain edema decrease using knockdown technique
Project/Area Number |
15K10526
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Anesthesiology
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Research Institution | Aichi Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
浅井 清文 名古屋市立大学, 大学院医学研究科, 教授 (70212462)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 脳低温 / アストロサイt / アクアポリン / 水チャネル / アストロサイト / 脳浮腫 / ノックダウン / AQP4 |
Outline of Final Research Achievements |
For making aquaporin (AQP) 4 knock down, we used Lentiviral RNAi system of Invitrogen corporation. Finally, we obtained AQP 4 knockdown model with pLenti4/BLOCK-IT/Expression Construct and plasmid of pLP1、pLP2、pLP/VSVG、and pENTER-gus. We confirmed that AQP4 knockdown that was stronger than our earlier experiment. We constructed hAQP4-M23 and hAQP4-M1 vector and transfected into C6 cells. We confirmed the overexpression of hAQP4-M1 and hAQP4-M23 in C6 cells. Moreover, we constructed permanent cell line of hAQP4-M23-overexpressed-cells of C6.In hypoxic condition, we tried to decide apoptosis or necrosis with flow cytometry. For optimal oxygen concentration, we experimented cell culture of astrocyte and SH-SY5Y in hyperoxia. We confirmed cell damage of hyperoxia in this condition. We try to continue neuron protection of various condition and drugs using RNAi technique.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Cardiac arrest caused by rapidly increasing ascites in a patient with TAFRO syndrome: a case report2017
Author(s)
5.Masatoshi Okumura, Atsushi Ujiro, Yasunori Otsuka, Hiroshi Yamamoto, Sho Wada, Hirofumi Iwata, Toshiaki Kan, Seiji Miyauchi, Atsushi Hashimoto, Yuko Sato, Yoshihito Fujita, Yoshihiro Fujiwara, Hideki Shimaoka
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Journal Title
Acute Medicine & Surgery
Volume: 4
Pages: 344-348
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] ardiac arrest caused by rapidly increasing ascites in a patient with TAFRO syndrome: a case report2017
Author(s)
Masatoshi Okumura, Atsushi Ujiro, Yasunori Otsuka, Hiroshi Yamamoto, Sho Wada, Hirofumi Iwata, Toshiaki Kan, Seiji Miyauchi, Atsushi Hashimoto, Yuko Sato, Yoshihito Fujita, Yoshihiro Fujiwara, Hideki Shimaoka.
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Journal Title
Acute Medicine & Surgery
Volume: 印刷中
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Estimation of the success rate of anesthetic management for thymectomy in patients with myasthenia gravis treated without muscle relaxants: A retrospective observational cohort study2015
Author(s)
Yoshihito Fujita, Satoru Moriyama, Satoshi Aoki, Saya Yoshizawa, Maiko Tomita, Taiki Kojima, Yukiko Mori, Naoko Takeuchi, Min-Hye So, Motoki Yano, Kazuya Sobue
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Journal Title
Journal of Anesthesia
Volume: 29
Issue: 5
Pages: 794-797
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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